Quantitative and qualitative analysis of rhinovirus infection in bronchial tissues
Quantitative and qualitative analysis of rhinovirus infection in bronchial tissues
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DOI：
10.1164/rccm.200407-970oc
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发表时间：
2005-03-15
影响因子：
24.7
通讯作者：
Gern, JE
中科院分区：
文献类型：
--
作者：
Mosser, AG;Vrtis, R;Gern, JE
虽然鼻病毒（RV）感染可导致哮喘恶化并改变下气道炎症和生理学，但支气管感染对这些过程的重要性尚不清楚。为了研究急性感染期间下呼吸道组织中RV出现的动力学、位置和频率，采用免疫组织化学和定量聚合酶链反应分析了19例实验性RV血清型16（RV 16）感冒受试者的鼻灌洗液、痰液、支气管肺泡灌洗液、支气管刷拭和活检标本中细胞中病毒的存在。通过聚合酶链反应分析，所有受试者接种RV 16后的鼻灌洗液和诱导痰样本中的细胞，以及接种病毒后4天的19例支气管肺泡灌洗液细胞样本中的5例和18例支气管活检样本中的5例，均检测到RV。免疫组化检测RV16在39和36%的所有活检和刷取样品接种后4和15天，分别。感染细胞主要分布在上皮上的离散补丁。这些结果证实了下呼吸道组织的感染是感冒期间的常见现象，并进一步证明了受感染细胞的斑片状分布，该模式与上呼吸道组织中报道的模式相似。
Although rhinovirus (RV) infections can cause asthma exacerbations and alter lower airway inflammation and physiology, it is unclear how important bronchial infection is to these processes. To study the kinetics, location, and frequency of RV appearance in lower airway tissues during an acute infection, immunohistochemistry and quantitative polymerase chain reaction analysis were used to analyze the presence of virus in cells from nasal lavage, sputum, bronchoalveolar lavage, bronchial brushings, and biopsy specimens from 19 subjects with an experimental RV serotype 16 (RV16) cold. RV was detected by polymerase chain reaction analysis on cells from nasal lavage and induced sputum samples from all subjects after RV16 inoculation, as well as in 5 of 19 bronchoalveolar lavage cell samples and in 5 of 18 bronchial biopsy specimens taken 4 days after virus inoculation. Immunohistochemistry detected RV16 in 39 and 36% of all biopsy and brushing samples taken 4 and 15 days, respectively, after inoculation. Infected cells were primarily distributed in discrete patches on the epithelium. These results confirm that infection of lower airway tissues is a frequent finding during a cold and further demonstrate a patchy distribution of infected cells, a pattern similar to that reported in upper airway tissues.