Sequence variation in TgROP7 gene among Toxoplasma gondii isolates from different hosts and geographical regions
Sequence variation in TgROP7 gene among Toxoplasma gondii isolates from different hosts and geographical regions
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DOI：
10.5897/ajb12.041
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发表时间：
2012-03
影响因子：
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通讯作者：
Y. Zhou;P. Lu;M. Xu;D. Ren;Dong‐Hui Zhou;Hx Li;R. Lin;F. Zou;Z. Yuan
中科院分区：
文献类型：
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作者：
Y. Zhou;P. Lu;M. Xu;D. Ren;Dong‐Hui Zhou;Hx Li;R. Lin;F. Zou;Z. Yuan
弓形虫病是世界范围内最常见的人畜共患寄生虫病之一，可感染哺乳动物和鸟类等多种宿主。本研究检测了不同类型T.不同宿主和地理位置的弓形虫分离株。系统发育分析表明，T.采用最大似然法（ML）检测弓形虫分离株。序列分析表明，在19个被检测的T.弓形虫分离株，对应的序列变异为0 ~ 1.7%，发生在第1、2、3密码子。系统发育分析表明，ROP 7基因在所研究的T. ROP 7序列不适合作为弓形虫的遗传标记。弓形虫分离株本研究结果表明，ROP 7基因可能是一个合适的疫苗候选。关键词：序列变异，ROP 7基因，弓形虫，弓形虫病，系统发育分析。
Toxoplasma gondii can infect a wide range of hosts including mammals and birds, causing toxoplasmosis which is one of the most common parasitic zoonoses worldwide. The present study examined sequence variation in rhoptry 7 (ROP7) gene among different T. gondii isolates from different hosts and geographical localities. Phylogenetic analysis of the examined T. gondii isolates was conducted using the maximum likelihood (ML) method. Sequence analysis revealed that 60 nucleotide positions were variable in the ROP7 gene sequences among the 19 examined T. gondii isolates, corresponding to sequence variations of 0 to 1.7%, which occurred at the first, second and third codons. Phylogenetic analysis indicated that sequence variation in ROP7 gene was low among the examined T. gondii isolates from different hosts and geographical localities, and that the ROP7 sequence was not suitable as genetic marker for the differentiation of T. gondii isolates. The results of the present study suggest that ROP7 gene may be a suitable vaccine candidate. Key words : Sequence variation, rhoptry 7 (ROP7) gene, Toxoplasma gondii , toxoplasmosis, phylogenetic analysis.