ACCUMULATION OF RNA HOMOLOGOUS TO HUMAN PAPILLOMAVIRUS TYPE-16 OPEN READING FRAMES IN GENITAL PRECANCERS
ACCUMULATION OF RNA HOMOLOGOUS TO HUMAN PAPILLOMAVIRUS TYPE-16 OPEN READING FRAMES IN GENITAL PRECANCERS
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DOI：
10.1128/jvi.62.1.84-90.1988
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发表时间：
1988-01-01
影响因子：
5.4
通讯作者：
SILVERSTEIN, SJ
中科院分区：
文献类型：
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作者：
CRUM, CP;NUOVO, G;SILVERSTEIN, SJ
用单链35s标记RNA原位杂交技术研究了人乳头瘤病毒16型（HPV-16）特异性RNA在生殖器癌前病变患者活检组织切片中的积累。这些分析表明，最丰富的早期区域rna来自E4和E5开放阅读框（orf）。与E6/E7 ORF同源的rna也被检测到，而与中间的E1 ORF同源的rna则未被检测到。这表明E4和E5 mrna是通过剪接到上游的E6/E7 orf而衍生的，这与HPV-11在尖锐湿疣中的研究一致（L. T. Chow等人，Cancer Cells (Cold Spring Harbor) 5:55-72, 1987）。同时还检测到大量与L1的5”部分同源的rna。这些rna定位于上皮的顶端层。HPV-16 rna在这些病变的离散区域积累，当存在时，在癌前上皮的上层细胞层中最丰富。在上皮基底层的一些生发细胞中也检测到与早期orf同源的rna。
The accumulation of human papillomavirus type 16 (HPV-16)-specific RNAs in tissue sections from biopsies of patients with genital precancers was studied by in situ hybridization with single-stranded 35S-labeled RNA. These analyses revealed that the most abundant early-region RNAs were derived from the E4 and E5 open reading frames (ORFs). RNAs homologous to the E6/E7 ORFs were also detected, whereas RNAs homologous to the intervening E1 ORF were not. This suggests that the E4 and E5 mRNAs are derived by splicing to the upstream E6/E7 ORFs, consistent with studies of HPV-11 in condylomata (L. T. Chow et al., Cancer Cells (Cold Spring Harbor) 5:55-72, 1987). Abundant RNAs homologous to the 5'' portion of L1 were also detected. These RNAs were localized to the apical strata of the epithelium. HPV-16 RNAs accumulated in discrete regions of these lesions, and when present were most abundant in the upper cell layers of the precancerous epithelium. RNAs homologous to early ORFs were also detected in some germinal cells within the basal layer of the epithelium.