Immunohistochemical Expression of Hsp60 Correlates With Tumor Progression and Hormone Resistance in Prostate Cancer
Immunohistochemical Expression of Hsp60 Correlates With Tumor Progression and Hormone Resistance in Prostate Cancer
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DOI：
10.1016/j.urology.2010.05.045
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发表时间：
2010-10-01
期刊：
影响因子：
2.1
通讯作者：
Saez, Carmen
中科院分区：
文献类型：
--
作者：
Castilla, Carolina;Congregado, Belen;Saez, Carmen
目的探讨前列腺癌活检组织中热休克蛋白60(Hsp60)的表达及其与临床预后参数、激素抵抗和生存的关系。分子伴侣参与蛋白质折叠、蛋白质降解和蛋白质在亚细胞间的转运。方法我们选择了1999年至2004年在本院就诊的107例局限性和局部晚期前列腺癌患者。对石蜡包埋组织切片进行免疫印迹和免疫组织化学分析。结果Gleason评分高的肿瘤组织中HSP60的表达水平显著升高(P<；.001)。Hsp60的表达还与初始血清PSA水平(P<；.01)和有无淋巴结转移(P<；.003)显著相关。在50例接受雄激素治疗的局部晚期癌症中，我们发现Hsp60高表达的肿瘤与激素难治性疾病的早期发病(P<；0.02)和癌症特异性生存率降低(P<；0.05)有关。结论Hsp60蛋白在低分化前列腺癌中过度表达。HSP60的表达与预后的临床参数，如Gleason评分、初始血清PSA水平和淋巴结转移密切相关，并与激素难治性疾病的发生和癌症特异性生存期的降低密切相关。这些标记物的鉴定在前列腺癌的临床治疗中可能是相关的。泌尿外科76：1017.e1-1017.e6,2010。(C)2010年爱思唯尔公司。
OBJECTIVES To investigate the expression of Hsp60 protein in prostate cancer biopsy samples, and its association with prognostic clinical parameters and hormone resistance and survival. Molecular chaperones are involved in protein folding, protein degradation, and protein trafficking among subcellular compartments.METHODS We selected 107 patients with localized and locally advanced prostate cancer at our hospital from 1999 through 2004. We performed an analysis by western blot and immunohistochemistry on paraffin-embedded tissue sections. Clinical data were used to determine associations between immunohistochemical expression of Hsp60 and tumor behavior.RESULTS The expression level of Hsp60 was significantly increased in tumors with high Gleason score (P < .001). Hsp60 expression was also significantly associated with initial serum PSA levels (P < .01) and with the presence of lymph node metastasis (P < .003). In 50 locally advanced cancers treated by androgen ablation we found an association between high Hsp60-expressing tumors and an early onset of hormone refractory disease (P < .02) and reduced cancer-specific survival (P < .05).CONCLUSIONS Hsp60 protein is overexpressed in poorly differentiated prostate cancers. Hsp60 expression is strongly associated with prognostic clinical parameters, such as Gleason score, initial serum PSA levels, and lymph node metastasis and with the onset of hormone-refractory disease and reduced cancer-specific survival. Identification of such markers could be of relevance in the clinical management of prostate cancer. UROLOGY 76: 1017.e1-1017.e6, 2010. (C) 2010 Elsevier Inc.