HPLC simultaneous determination of arbutin, chlorogenic acid and 6′-O-caffeoylarbutin in different parts of Vaccinium dunalianum Wight
HPLC simultaneous determination of arbutin, chlorogenic acid and 6′-O-caffeoylarbutin in different parts of Vaccinium dunalianum Wight
复制标题
DOI：
10.1080/14786419.2015.1013472
复制
发表时间：
2015-10-18
影响因子：
2.2
通讯作者：
Zhang, Ying-Jun
中科院分区：
文献类型：
--
作者：
Luo, Xu-Lu;Li, Na;Zhang, Ying-Jun
熊果苷(1)、绿原酸(2)和6′- o -咖啡熊果苷(3)是双柳中的三种主要成分，具有多种生物活性。建立了一种可靠、重复性好、准确的同时定量测定1-3的RP-HPLC分析方法。该方法适用于双歧杆菌未加工品和加工品的质量保证。分析了不同产地杜鹃不同部位中1-3的含量。3的含量远高于1和2，在干叶芽中的含量高达31.76%。叶芽、花蕾和叶片中1-3的含量高于其他部位。
Arbutin (1), chlorogenic acid (2) and 6 '-O-caffeoylarbutin (3) are three major components in Vaccinium dunalianum Wight with various promising bioactivities. A reliable, reproducible and accurate method for simultaneous and quantitative determination of 1-3 is developed by RP-HPLC analysis. This method should be appropriate for the quality assurance of unprocessed and processed materials of V. dunalianum. The contents of 1-3 in different parts of V. dunalianum from different origins were analysed. The content of 3 was much higher than those of 1 and 2, accounting for up to 31.76% in the dried leaf buds. Moreover, the leaf buds, flower buds and leaves showed a tendency towards higher contents of 1-3 than the other plant parts.