Endoplasmic reticulum stress pathway mediates the early heat stress response of developing rice seeds
Endoplasmic reticulum stress pathway mediates the early heat stress response of developing rice seeds
复制标题
内质网应激途径介导发育中水稻种子的早期热应激反应
DOI：
10.1111/pce.14103
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Walia, Harkamal
中科院分区：
文献类型：
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作者：
Sandhu, Jaspreet;Irvin, Larissa;Liu, Kan;Staswick, Paul;Zhang, Chi;Walia, Harkamal
水稻种子发育早期的短暂热胁迫通过改变胚乳发育而减小种子大小。然而，应激时间和特定发育阶段对热敏感性的影响之间的关系尚未得到很好的理解。为了解决这个问题，我们实施了一系列非重叠的热胁迫处理，发现年轻的种子在开花后的前两天最敏感。在此窗口期间，发育中的热应激（35°C）种子的时间转录组分析表明，肌醇需要酶1（IRE 1）介导的内质网（ER）应激反应和茉莉酸（JA）途径是热应激反应的早期（1-3 h）驱动因素。我们认为，热应激下JA水平的增加可能先于ER应激反应，因为JA应用促进了OsbZIP 50的剪接形式，OsbZIP 50是与IRE 1特异性通路相关的ER应答标记基因。本研究提出了时间和机制的见解JA和ER胁迫信号的作用，在早期热胁迫反应的水稻种子，影响籽粒大小和品质。调控早期热敏感途径和下游胚乳发育基因的热敏感性可以增强水稻对瞬时热胁迫事件的恢复力。
A transient heat stress occurring during early seed development in rice (Oryza sativa) reduces seed size by altering endosperm development. However, the relationship between the timing of the stress and specific developmental stage on heat sensitivity is not well‐understood. To address this, we imposed a series of non‐overlapping heat stress treatments and found that young seeds are most sensitive during the first two days after flowering. Temporal transcriptome analysis of developing, heat stressed (35°C) seeds during this window shows thatInositol‐requiring enzyme 1 (IRE1)‐mediated endoplasmic reticulum (ER) stress response and jasmonic acid (JA) pathways are the early (1–3 h) drivers of heat stress response. We propose that increased JA levels under heat stress may precede ER stress response as JA application promotes the spliced form ofOsbZIP50,an ER response marker gene linked to IRE1‐specific pathway. This study presents temporal and mechanistic insights into the role of JA and ER stress signalling during early heat stress response of rice seeds that impact both grain size and quality. Modulating the heat sensitivity of the early sensing pathways and downstream endosperm development genes can enhance rice resilience to transient heat stress events.