Identification of glutamic acid 113 as the Schiff base proton acceptor in the metarhodopsin II photointermediate of rhodopsin.
Identification of glutamic acid 113 as the Schiff base proton acceptor in the metarhodopsin II photointermediate of rhodopsin.
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鉴定谷氨酸 113 作为视紫红质变视紫红质 II 光中间体中希夫碱质子受体。
DOI：
10.1021/bi00202a005
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发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
F. Siebert
中科院分区：
文献类型：
--
作者：
F. Jaeger;K. Fahmy;T. Sakmar;F. Siebert
In order to investigate the molecular mechanism of rhodopsin photoactivation, site-directed mutants of bovine rhodopsin were studied by Fourier-transform infrared (FTIR) difference spectroscopy. Rhodopsin mutants E113D and E113A were prepared in which the retinylidene Schiff base counterion, Glu113, was replaced by Asp and Ala, respectively. FTIR difference spectra were recorded and compared with spectra of recombinant native rhodopsin. Both mutant pigments formed photoproducts at 0 degrees C with vibrational absorption bands typical of the metarhodopsin II (MII) state of rhodopsin. The FTIR difference spectrum of E113D was nearly identical to that of rhodopsin. A positive band at 1712 cm-1 caused by the protonation of an internal carboxylic acid in rhodopsin was shifted slightly to 1709 cm-1 in mutant E113D. E113A was studied at acidic pH in the presence of chloride as an inorganic counterion to the protonated Schiff base. The 1712-cm-1 (1709-cm-1) band was absent in the FTIR difference spectrum of mutant E113A. Therefore, we have assigned the 1712-cm-1 absorbance band to the C = O stretching vibration of protonated Glu113 in MII of rhodopsin. These results show that the Schiff base counterion of rhodopsin, the carboxylate side chain of Glu113, becomes protonated during MII formation.
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视紫红质中视网膜质子化席夫碱抗衡离子的定位。
影响因子：
3.4
作者：
Han,M;DeDecker,BS;Smith,SO
通讯作者：
Smith,SO
视觉兴奋和恢复。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Stryer,L
通讯作者：
Stryer,L
影响因子：
56.9
作者：
E. Zhukovsky;D. Oprian
通讯作者：
E. Zhukovsky;D. Oprian
DOI：
10.1073/pnas.86.21.8309
发表时间：
1989-11-01
影响因子：
11.1
作者：
SAKMAR, TP;FRANKE, RR;KHORANA, HG
通讯作者：
KHORANA, HG
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Franke,RR;Sakmar,TP;Graham,RM;Khorana,HG
通讯作者：
Khorana,HG