Microglial reaction in MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) induced Parkinson's disease mice model
Microglial reaction in MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) induced Parkinson's disease mice model
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DOI：
10.1006/neur.1996.0020
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发表时间：
1996-06-01
期刊：
影响因子：
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通讯作者：
Czlonkowski, A
中科院分区：
文献类型：
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作者：
Czlonkowska, A;Kohutnicka, M;Czlonkowski, A
采用1-甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP）诱导的帕金森病（PD）模型，研究了小鼠小胶质细胞反应。通过Griffonia simplicifolia凝集素（GSA-1-B-4）鉴定小胶质细胞。多巴胺能神经元标记酪氨酸羟化酶抗体。中毒后第1 - 14天黑质和第1 - 4天纹状体细胞数量增加和形态学变化（凝集素染色增加、细胞体变大和突起变厚）表明小胶质细胞活化。多巴胺能神经元的耗竭在治疗后7天和14天最明显。结果表明，小胶质细胞的激活可能参与了一系列的病理变化，导致多巴胺能神经元损伤后MPTP中毒。(C)出版社：Academic Press Limited。
We studied the microglial reaction in mice using the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced model for Parkinson's disease (PD). Microglial cells were identified by means of the Griffonia simplicifolia lectin (GSA-I-B-4). Dopaminergic neurons were marked by tyrosine hydroxylase antibodies. Microglial activation was demonstrated by an increase in cellular number and changes of morphology (increased lectin staining, larger cell bodies and thicker processes) were seen in the substantia nigra from the 1st to the 14th day and in the striatum from the 1st to the 4th day after intoxication. Depletion of dopaminergic neurons was most pronounced 7 and 14 days following the treatment. The results suggest that microglial activation may be involved in the sequence of pathological changes that lead to dopaminergic neuronal damage after MPTP intoxication. (C) 1996 Academic Press Limited.