A novel reactive ester derivative of biotin with reduced membrane permeability for in vivo biotinylation experiments
A novel reactive ester derivative of biotin with reduced membrane permeability for in vivo biotinylation experiments
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DOI：
10.1002/pmic.201000308
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发表时间：
2010-10-01
期刊：
影响因子：
3.4
通讯作者：
Roesli, Christoph
中科院分区：
文献类型：
--
作者：
Strassberger, Verena;Truessel, Sabrina;Roesli, Christoph
The in vivo perfusion of rodent models of disease with biotin derivatives and the subsequent comparative proteomic analysis of healthy and diseased tissues represent a promising methodology for the identification of vascular accessible biomarkers. A novel, triply charged biotinylation reagent, NHS-beta-Ala-(L-Asp)(3)-biotin, was synthesized and validated in terms of its applicability for in vivo protein biotinylation. Compared to sulfo-NHS-LC-biotin, NHS-beta-Ala(L-Asp)(3)-biotin exhibited a reduced membrane permeability and a preferential labeling of proteins localized in compartments readily accessible in vivo from the vasculature.
