Response of the Pacific oyster Crassostrea gigas to hydrocarbon contamination under experimental conditions
Response of the Pacific oyster Crassostrea gigas to hydrocarbon contamination under experimental conditions
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DOI：
10.1016/j.gene.2003.12.027
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发表时间：
2004-03-31
期刊：
影响因子：
3.5
通讯作者：
Moraga, D
中科院分区：
文献类型：
--
作者：
Boutet, I;Tanguy, A;Moraga, D
碳氢化合物污染扰乱了海洋双壳类长牡蛎的代谢。为了了解这种生物对碳氢化合物暴露的反应，采用抑制减法杂交方法来表征碳氢化合物暴露过程中上调和下调的基因。该方法共获得258个差异表达基因序列。采用RT-PCR方法分析了参与烃解毒（细胞色素p4501a1样蛋白、细胞色素b(5)、含黄素单加氧酶2和谷胱甘肽s -转移酶omega类）、抗氧化应激（铜/锌超氧化物歧化酶）和细胞保护（热休克蛋白70家族）的基因表达。所有研究基因mRNA水平均升高。酶联免疫吸附试验（ELISA）对HSP70的定量分析显示，暴露期间该蛋白显著增加。本研究为研究海洋双壳类，特别是巨壳壳类的解毒过程提供了基础。(C) 2004 Elsevier b.v.版权所有
Hydrocarbon contamination perturbs the metabolism of the marine bivalve Crassostrea gigas. To understand the response of this organism to hydrocarbon exposure, a suppression subtractive hybridisation method was employed to characterise up- and down-regulated genes during hydrocarbon exposure. The number of differentially expressed gene sequences obtained via this method was 258. The expression of genes involved in hydrocarbon detoxification (cytochrome P4501A1-like protein, cytochrome b(5), flavin-containing monooxygenase 2 and glutathione S-transferase omega class), protection against oxidative stress (copper/zinc superoxide dismutase) and cell protection (heat shock protein 70 family) was analysed by RT-PCR. An increase in the mRNA level of all genes studied was observed. A quantification of HSP70 by Enzyme Linked Immunosorbent Assay (ELISA) showed a significant increase of this protein during exposure. This study provides a basis for studying hydrocarbon detoxification processes in marine bivalves, especially C. gigas. (C) 2004 Elsevier B.V All rights reserved.