Rice OsGL1-1 Is Involved in Leaf Cuticular Wax and Cuticle Membrane
Rice OsGL1-1 Is Involved in Leaf Cuticular Wax and Cuticle Membrane
复制标题
水稻OsGL1-1参与叶角质层蜡质和角质层膜的形成。
DOI：
10.1093/mp/ssr028
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发表时间：
2011-11-01
期刊：
影响因子：
27.5
通讯作者：
Cheng, Zhu-Kuan
中科院分区：
文献类型：
--
作者：
Qin, Bao-Xiang;Tang, Ding;Cheng, Zhu-Kuan
角质层蜡在气生植物器官上形成疏水屏障；它在保护植物免受多种形式的环境胁迫造成的损害方面发挥着重要作用。在本研究中，我们鉴定了源自自发突变的水稻叶蜡缺陷突变体 osgl1-1，该突变体表现出蜡缺陷和高度亲水的叶子表型。我们通过图位克隆方法克隆了OsGL1-1基因，并进行互补测试以确认候选基因的功能。分子研究表明，OsGL1-1 是 OsGL1 家族的成员，并且包含与甾醇去饱和酶和短链脱氢酶/还原酶中的某些区域同源的区域。与野生型相比，osgl1-1突变体表现出角质层蜡沉积减少、角质膜变薄、叶绿素淋溶减少、失水率增加、对干旱的敏感性增强。 OsGL1-1 在水稻中普遍表达。 OsGL1-1-绿色荧光蛋白融合蛋白的瞬时表达表明OsGL1-1定位于细胞质、质膜和细胞核。
Cuticular wax forms a hydrophobic barrier on aerial plant organs; it plays an important role in protecting a plant from damage caused by many forms of environmental stress. In the present study, we characterized a rice leaf wax-deficient mutant osgl1-1 derived from a spontaneous mutation, which exhibited a wax-deficient and highly hydrophilic leaf phenotype. We cloned the OsGL1-1 gene by the map-based cloning method and performed a complementation test to confirm the function of the candidate gene. Molecular studies revealed that OsGL1-1 was a member of the OsGL1 family, and contained regions that were homologous to some regions in sterol desaturases and short-chain dehydrogenases/reductases. Compared to the wild-type, the osgl1-1 mutant showed decreased cuticular wax deposition, thinner cuticular membrane, decreased chlorophyll leaching, increased rate of water loss, and enhanced sensitivity to drought. OsGL1-1 is expressed ubiquitously in rice. The transient expression of OsGL1-1-green fluorescent protein fusion protein indicated that OsGL1-1 is localized in the cytoplasm, plasma membrane, and nucleus.