Storage and Lyophilization of Pure Proteins
Storage and Lyophilization of Pure Proteins
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DOI：
10.1007/978-1-4939-6412-3_9
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发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Colliton, Keith
中科院分区：
文献类型：
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作者：
O'Fagain, Ciaran;Colliton, Keith
本文概述了储存纯蛋白质并保持高水平生物活性的经验程序。它描述了防止微生物污染和蛋白水解降解的简单且可行的方法，以及各种类型的稳定添加剂的使用。它阐述了冻干（也称为冷冻干燥，一个包括冷冻、初级染色和二级干燥阶段的复杂过程）的原理。以下是使用冻干机设备的一般程序，重点是最佳实践和要避免的陷阱。描述了使用调制差示扫描量热法测量玻璃化转变温度，玻璃化转变温度是冻干工艺设计和成功操作的关键参数。
This article outlines empirical procedures for the storage of pure proteins with preservation of high levels of biological activity. It describes simple and workable means of preventing microbial contamination and proteolytic degradation, and the use of various types of stabilizing additives. It sets out the principles of lyophilization (otherwise known as freeze-drying, a complex process comprising freezing, primary dying, and secondary drying stages). There follows a general procedure for the use of lyophilizer apparatus with emphasis on best practice and on pitfalls to avoid. The use of modulated differential scanning calorimetry to measure the glass transition temperature, a key parameter in the design and successful operation of lyophilization processes, is described.