Differentiated growth of human renal tubule cells on thin-film and nanostructured materials
Differentiated growth of human renal tubule cells on thin-film and nanostructured materials
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DOI：
10.1097/01.mat.0000205228.30516.9c
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发表时间：
2006-05-01
期刊：
影响因子：
4.2
通讯作者：
Roy, S
中科院分区：
文献类型：
--
作者：
Fissell, WH;Manley, S;Roy, S
超过 30 万美国人依靠血液透析来治疗肾衰竭，而肾移植则因供体器官稀缺而受到限制。这种短缺促使人们对肾脏替代疗法的组织工程进行研究。现有的生物人工肾体积较大，使用时劳动强度大，但在临床前研究和美国食品和药物管理局批准的 2 期临床试验中，与传统疗法相比，它们的生存率有所提高。这种混合技术需要血滤器、细胞培养基质、传感器的小型化以及控制电子设备的集成。使用与制备临床用生物人工肾相同的收获和分离技术，我们表征了人肾小管细胞在微机电系统（MEMS）构建中常用的各种硅和相关薄膜材料基底上的生长，以及新型硅纳米孔膜（SNM）。将人皮质管状上皮细胞 (HCTC) 接种到单晶硅、多晶硅、二氧化硅、氮化硅、SU-8 光刻胶、SNM 样品上。和聚酯组织培养插入物，并生长至汇合。细胞形成具有紧密连接和中央纤毛的汇合单层。 SNM 和聚酯膜的跨上皮电阻相似。人肾小管上皮细胞在MEMS材料上的分化生长强烈表明现有生物人工肾的小型化是可行的，为这项新技术的广泛应用铺平了道路。
Over 300,000 Americans are dependent on hemodialysis as treatment for renal failure, and kidney transplantation is limited by scarcity of donor organs. This shortage has prompted research into tissue engineering of renal replacement therapy. Existing bioartificial kidneys are large and their use labor intensive, but they have shown improved survival compared to conventional therapy in preclinical studies and an US Food and Drug Administration-approved phase 2 clinical trial. This hybrid technology will require miniaturization of hemofilters, cell culture substrates, sensors, and integration of control electronics. Using the same harvesting and isolation techniques used in preparing bioartificial kidneys for clinical use, we characterized human renal tubule cell growth on a variety of silicon and related thin-film material substrates commonly used in the construction of microelectromechanical systems (MEMS), as well as novel silicon nanopore membranes (SNMs). Human cortical tubular epithelial cells (HCTC) were seeded onto samples of single-crystal silicon, polycrystalline silicon, silicon dioxide, silicon nitride, SU-8 photoresist, SNMs. and polyester tissue culture inserts, and grown to confluence. The cells formed confluent monolayers with tight junctions and central cilia. Transepithelial resistances were similar between SNMs and polyester membranes. The differentiated growth of human tubular epithelial cells on MEMS materials strongly suggests that miniaturization of the existing bioartificial kidney will be feasible, paving the way for widespread application of this novel technology.