RhoB regulates endosome transport by promoting actin assembly on endosomal membranes through Dia1
RhoB regulates endosome transport by promoting actin assembly on endosomal membranes through Dia1
复制标题
DOI：
10.1242/jcs.02384
复制
发表时间：
2005-06-15
影响因子：
4
通讯作者：
Neefjes, J
中科院分区：
文献类型：
--
作者：
Fernandez-Borja, M;Janssen, L;Neefjes, J
Rho gtpase是肌动蛋白细胞骨架的重要调节因子，它们在控制膜运输中发挥作用。与家族成员RhoA和RhoC相反，RhoB定位于核内体并延迟表皮生长因子受体的转运。在这里，我们发现活化的RhoB诱导核内体的外周分布，核内体沿着皮质下肌动蛋白应力纤维排列，并被肌动蛋白外壳包围。与蝶状体相关的双胍Dial通过活化的RhoB被招募到核内体中。Dial是RhoB下游核内体周围肌动蛋白外壳形成所必需的，将膜运输与肌动蛋白动力学调节联系起来。
Rho GTPases are crucial regulators of the actin cytoskeleton and they play a role in the control of membrane trafficking. In contrast to the close family members RhoA and RhoC, RhoB localises to endosomes and delays epidermal growth factor receptor traffic. Here, we show that activated RhoB induces the peripheral distribution of endosomes, which align along subcortical actin stress fibres and are surrounded by an actin coat. The Diaphanous-related formin, Dial, is recruited to endosomes by activated RhoB. Dial is required for the formation of the actin coat around endosomes downstream of RhoB, connecting membrane trafficking with the regulation of actin dynamics.