Comparison between adenosine and isoflurane for assessing the coronary flow reserve in mouse models of left ventricular pressure and volume overload
Comparison between adenosine and isoflurane for assessing the coronary flow reserve in mouse models of left ventricular pressure and volume overload
复制标题
腺苷和异氟烷评估左心室压力和容量超负荷小鼠模型冠状动脉血流储备的比较
DOI：
10.1152/ajpheart.00612.2012
复制
发表时间：
2012-11-01
影响因子：
4.8
通讯作者：
Zou, Yunzeng
中科院分区：
文献类型：
--
作者：
You, Jieyun;Wu, Jian;Zou, Yunzeng
You J，Wu J，GeJ，Zou Y.腺苷和异氟醚对左心室压力和容量超负荷小鼠模型冠状动脉血流储备的比较。Am J Physiol Heart Circ Physiol 303：H1199-H1207，2012。首次发表于2012年9月21日; doi：10.1152/ajpheart.00612.2012.-腺苷和高浓度异氟醚常用于诱导充血以评估小鼠的冠状动脉血流储备（CFR），但高浓度异氟醚可能会加重心功能不全，导致CFR受损。但是，目前还没有研究比较腺苷和异氟醚对CFR和相应心功能的影响。20只小鼠在主动脉缩窄（TAC）、主动脉瓣关闭不全（AR）和相应的假手术后2周进行高分辨率超声心动图和有创血流动力学评估。CFR计算为充血与基础舒张期峰值速度（CFRpdv）或舒张期速度-时间积分（CFRdvti）的比值。在假手术小鼠中，未观察到腺苷和异氟烷对CFR、左心室收缩功能（左心室射血分数和缩短分数）、左心室收缩末期压、最大收缩和舒张速度（+dp/dt和-dp/dt）、左心室压改变（Delta LVP）或+/- dp/dt（Delta dp/dt）的影响存在差异。但腺苷衍生的结果显着高于异氟烷衍生的TAC和AR组。CFRpdv或CFRdvti与LVEF和LVFS均呈正相关。与腺苷衍生的CFR相比，异氟烷衍生的CFR在TAC和AR小鼠中可能被低估，这可能与心脏功能抑制有关。
You J, Wu J, Ge J, Zou Y. Comparison between adenosine and isoflurane for assessing the coronary flow reserve in mouse models of left ventricular pressure and volume overload. Am J Physiol Heart Circ Physiol 303: H1199-H1207, 2012. First published September 21, 2012; doi: 10.1152/ajpheart.00612.2012.-Adenosine and high-concentration isoflurane are commonly used to induce hyperemia for assessment of coronary flow reserve (CFR) in mice, but high-concentration isoflurane may exacerbate cardiac dysfunction, leading to impaired CFR. However, there is no study be found comparing the effects of adenosine and isoflurane on CFR and corresponding cardiac function. High-resolution echocardiography and invasive hemodynamic assessment were performed in 20 mice 2 wk after transverse aortic constriction (TAC), aortic regurgitation (AR), and corresponding sham operation. CFR was calculated as the ratio of hyperemic to basal peak diastolic velocity (CFRpdv) or diastolic velocity-time integral (CFRdvti). In the sham-operated mice, no differences were observed between the effects of adenosine and isoflurane on CFR, left ventricular systolic function (left ventricular ejection fraction and fractional shortening), left ventricular end-systolic pressure, maximal contraction and relaxation velocity (+dp/dt and -dp/dt), alteration of left ventricular pressure (Delta LVP), or +/- dp/dt (Delta dp/dt). But adenosine-derived results were significantly higher than isoflurane-derived ones in both the TAC and the AR groups. Moreover, CFRpdv or CFRdvti was positively correlated with both LVEF and LVFS. Compared with adenosine-derived CFR, isoflurane-derived CFR may be underestimated in the TAC and the AR mice, which is probably associated with suppressed cardiac function.