GLYCOPROTEIN COAT OF TA3 CELL - ISOLATION AND PARTIAL CHARACTERIZATION OF A SIALIC-ACID CONTAINING GLYCOPROTEIN FRACTION

GLYCOPROTEIN COAT OF TA3 CELL - ISOLATION AND PARTIAL CHARACTERIZATION OF A SIALIC-ACID CONTAINING GLYCOPROTEIN FRACTION
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DOI:
10.1021/bi00764a001
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发表时间:
1972-01-01
期刊:
影响因子:
2.9
通讯作者:
JEANLOZ, RW
JEANLOZ, RW
中科院分区:
生物学3区
文献类型:
--
作者:
CODINGTON, JF;SANFORD, BH;JEANLOZ, RW

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John F. Codington,* Barbara H. Sanford, and Roger W. Jeanloz abstract: Sialic acid containing glycoprotein fragments were removed from the surface of the TA3 mammary adenocar-cinoma ascites cell of the strain A mouse by short-term incu-bations with TPCK-trypsin at 4. The material eluted in the void volume of a Bio-Gel P-100 column, after fractionation on Bio-Gels P-4 and P-30, represented 9% of the carbohydrate and protein material removed from the cells but 31% of the total surface sialic acid. It was composed of approximately 70% carbohydrate and 30% protein and contained four carbo-