SIZE HETEROGENEITY AMONG ANTIGENICALLY RELATED GIARDIA-LAMBLIA VARIANT-SPECIFIC SURFACE-PROTEINS IS DUE TO DIFFERENCES IN TANDEM REPEAT COPY NUMBER
SIZE HETEROGENEITY AMONG ANTIGENICALLY RELATED GIARDIA-LAMBLIA VARIANT-SPECIFIC SURFACE-PROTEINS IS DUE TO DIFFERENCES IN TANDEM REPEAT COPY NUMBER
复制标题
DOI：
10.1128/iai.62.4.1213-1218.1994
复制
发表时间：
1994-04-01
影响因子：
3.1
通讯作者：
NASH, TE
中科院分区：
文献类型：
--
作者：
MOWATT, MR;NGUYEN, BYT;NASH, TE
贾第鞭毛虫通过调节构成滋养体变体特异性表面蛋白 (VSP) 库的不同基因的表达来经历抗原变异。我们研究了由来自独立的兰氏兰氏杆菌分离株 WB、G3M、Be-2 和 CAT 的克隆滋养体系表达的 VSP 中保守的表位。单克隆抗体 6E7 识别的表位完全位于串联重复的 65 个氨基酸单元区域内，这是这些大小变异 VSP 的特征。 Northern (RNA) 杂交、cDNA 克隆和 DNA 序列分析表明，这些 VSP 之间的大小异质性是由于重复单元数量的差异造成的。
Giardia lamblia undergoes antigenic variation by modulating the expression of the different genes that comprise the trophozoite's variant-specific surface protein (VSP) repertoire. We studied an epitope that is conserved among VSPs expressed by cloned trophozoite lines derived from the independent G. lamblia isolates WB, G3M, Be-2, and CAT. The epitope recognized by monoclonal antibody 6E7 lies entirely within the region of tandemly repeated 65-amino-acid units that is characteristic of these size-variant VSPs. Northern (RNA) hybridization, cDNA cloning, and DNA sequence analysis indicate that size heterogeneity among these VSPs is due to differences in the number of repetitive units.