Markers of the sympathetic, parasympathetic and sensory nervous system are altered in the human diabetic choroid
Markers of the sympathetic, parasympathetic and sensory nervous system are altered in the human diabetic choroid
复制标题
DOI：
10.1016/j.peptides.2021.170661
复制
发表时间：
2021-09-29
期刊：
影响因子：
3
通讯作者：
Sun, Xu-Fang
中科院分区：
文献类型：
--
作者：
Qin, Yuan-Jun;Xiao, Ke;Sun, Xu-Fang
背景：我们试图评估糖尿病患者脉络膜自主神经系统标志物的改变。方法：对18例糖尿病患者和22例非糖尿病患者的眼球进行了检查。突触素、酪氨酸羟化酶（TH）、多巴胺β-羟化酶（D8 H）、神经元型一氧化氮合酶（nNOS）、胆碱乙酰转移酶（ChAT）、囊泡单胺转运蛋白II（VMAT-2）、囊泡乙酰胆碱转运蛋白（VAChT）、血管活性肠肽（VIP）、神经肽Y（NPY）、用免疫印迹法检测降钙素基因相关肽（CGRP）水平，部分病例用免疫荧光法检测。此外，糖尿病受试者和对照组之间的肾上腺素能（α 1-和82-亚型）和胆碱能（M1和M3）受体水平的差异。结果如下：通过免疫印迹分析发现糖尿病脉络膜中突触素水平降低，免疫荧光法也发现突触素免疫反应神经减少。此外，去甲肾上腺素的关键酶（TH）和转运蛋白（VMAT 2）的水平下降是明显的，通过蛋白质印迹分析和免疫荧光。此外，在糖尿病脉络膜中观察到NPY、VAChT、nNOS和CGRP水平升高。糖尿病脉络膜中肾上腺素能（82亚型）和乙酰胆碱（M1亚型）受体的水平降低，如蛋白质印迹法所示，尽管α 1和M3的差异不显著，但有下降趋势。结论：在糖尿病脉络膜中，与脉络膜血流调节相关的神经递质、酶和受体的水平改变。这些变化可能影响脉络膜血流的调节，并可能与受损的视网膜功能和视网膜病理学有关。
Background: We sought to evaluate alterations in markers of the autonomic nervous system in human diabetic choroid. Methods: Eighteen eyeballs from subjects with diabetes and 22 eyeballs from subjects without diabetes were evaluated in this study. Synaptophysin, tyrosine hydroxylase (TH), dopamine beta-hydroxylase (D8H), neuronal nitric oxide synthase (nNOS), choline acetyltransferase (ChAT), vesicular monoamine transporter II (VMAT-2), vesicular acetylcholine transporter (VAChT), vasoactive intestinal peptide (VIP), neuropeptide Y (NPY), and calcitonin gene-related peptide (CGRP) levels were detected by western blot analysis and immunofluorescence was performed in some cases. Furthermore, differences in adrenergic (alpha 1-and 82-subtypes) and cholinergic (M1 and M3) receptor levels between diabetic subjects and controls were noted. Results: Decreased synaptophysin levels were found in diabetic choroids by western blot analysis and a reduction of synaptophysin-immunoreactive nerves was also found by immunofluorescence. Furthermore, a decrease of the levels of the key enzyme (TH) and transporter (VMAT2) of norepinephrine was evident both by western blot analysis and immunofluorescence. Additionally, increased NPY, VAChT, nNOS, and CGRP levels were observed in diabetic choroids. The levels of adrenergic (82 subtype) and acetylcholine (M1 subtype) receptors decreased in diabetic choroids, as shown by western blotting and although the differences in alpha 1 and M3 were not significant, there was a downward trend. Conclusions: In the diabetic choroid, the levels of neurotransmitters, enzymes, and receptors associated with choroidal blood flow regulation are altered. These changes may affect the regulation of choroidal blood flow and may be associated with impaired retinal function and retinal pathology.