A novel mechanism of vascular endothelial growth factor, leptin and transforming growth factor-beta2 sequestration in a subpopulation of human ovarian follicle cells.
A novel mechanism of vascular endothelial growth factor, leptin and transforming growth factor-beta2 sequestration in a subpopulation of human ovarian follicle cells.
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血管内皮生长因子、瘦素和转化生长因子-β2 在人卵巢卵泡细胞亚群中隔离的新机制。
DOI：
10.1093/humrep/12.10.2226
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Clark,A
中科院分区：
文献类型：
--
作者：
Antczak,M;VanBlerkom,J;Clark,A
本研究描述了一个高度专业化的颗粒细胞和卵丘细胞亚群的发生，积累和隔离特定的生长因子的一种新的机制。这些细胞的特征是多个气球样突起，通过一个细长的质膜柄与细胞相连。延时分析表明，这些栓系结构（TS）的形式在几分钟内，经常脱离细胞与球根状部分保持在细胞表面上的运动。透射电子显微镜图像的连续切片重建显示了一个特定的和稳定的细胞内组织，其中一个明显的分泌室由密集的空泡，囊泡和池组成，由一个厚的丝状网络从含有线粒体，多聚核糖体，脂质包涵体和粗糙表面内质网的核室分离。在这些结构的形成过程中进行的免疫荧光分析表明，血管内皮生长因子，瘦素，和转化生长因子-β 2在球根状区域的进行性积累。TS被确定在新吸出的群众的颗粒和卵丘oophorus，和他们的生产持续数月的文化。在培养的几天内对TS形成细胞的观察表明，它们的产生是间歇性的，并且这些细胞的因子释放可能是脉动的。研究结果表明，一种新的方法，生长因子的储存和释放的一个明显的apocrine样机制发生在人类卵巢卵泡。结果进行了讨论，在排卵前和排卵后卵泡发育的可能作用。
This study describes the occurrence of a highly specialized subpopulation of granulosa and cumulus oophorus cells that accumulate and sequester specific growth factors by a novel mechanism. These cells are characterized by multiple balloon-like processes tethered to the cell by means of a slender stalk of plasma membrane. Time-lapse analyses demonstrate that these tethered structures (TS) form in minutes and frequently detach from the cell with the bulbous portion remaining motile on the cell surface. Serial section reconstruction of transmission electron microscopic images shows a specific and stable intracellular organization in which an apparent secretory compartment composed of densely packed vacuoles, vesicles, and cisternae is separated by a thick filamentous network from a nuclear compartment containing mitochondria, polyribosomes, lipid inclusions, and rough-surfaced endoplasmic reticulum. Immunofluorescent analysis performed during the formation of these structures showed a progressive accumulation of vascular endothelial growth factor, leptin, and transforming growth factor-beta2 in the bulbous region. TS were identified in newly aspirated masses of granulosa and cumulus oophorus, and their production persists for months in culture. Observations of TS-forming cells made over several days of culture indicates that their production is episodic and factor release from these cells may be pulsatile. The findings suggest that a novel method of growth factor storage and release by an apparent apocrine-like mechanism occurs in the human ovarian follicle. The results are discussed with respect to possible roles in pre- and post-ovulatory follicular development.