A computerized infusion pump for control of tissue tracer concentration during positron emission tomography in vivo pharmacokinetic/pharmacodynamic measurements.
A computerized infusion pump for control of tissue tracer concentration during positron emission tomography in vivo pharmacokinetic/pharmacodynamic measurements.
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DOI：
10.1186/1756-6649-8-2
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发表时间：
2008-05-30
期刊：
影响因子：
--
通讯作者：
Bergstrom, Mats
中科院分区：
文献类型：
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作者：
Eriksson, Olof;Wallberg, Andreas;Bergstrom, Mats
背景：计算机控制输注泵(UIPump)用于调节放射性化合物的靶组织浓度，用于生物研究和PET示踪剂的开发。方法：根据示踪剂团注后观察到的组织或血浆动力学，算法计算获得特定靶点动力学曲线所需的输注量。计算机将这种输液方案输入通过静脉导管连接到动物身上的输液泵。用[11C]氟马西尼注射给Spraogue-Dawley大鼠，用微型PET扫描仪测量示踪剂随时间的全脑分布和动力学，验证了这一概念。通过产生示踪剂的稳态水平和口服给药后的模拟动力学来评估系统的准确度和精密度。结果：可以产生各种动力学曲线，包括快速达到恒定水平或逐步增加水平。所得到的组织曲线与目标曲线的偏差较小：AUC(%)：4.2+/-2.8，最大偏差(%)：13.6+/-5.0，R2：0.95+/-0.02。结论：UIPump系统适合于PET研究，通过模拟体内不同示踪剂的组织动力学来评估PK/PD的性质。
BACKGROUND: A computer controlled infusion pump (UIPump) for regulation of target tissue concentration of radioactive compounds was developed for use in biological research and tracer development for PET.METHODS: Based on observed tissue or plasma kinetics after a bolus injection of the tracer an algorithm calculates the infusion needed to obtain a specified target kinetic curve. A computer feeds this infusion scheme into an infusion pump connected to an animal via a venous catheter. The concept was validated using [11C]Flumazenil administrated to Sprague-Dawley rats where the whole brain distribution and kinetic of the tracer was measured over time using a microPET-scanner. The accuracy and precision of the system was assessed by producing steady-state levels of the tracer and by mimicking kinetics after oral administration.RESULTS: Various kinetic profiles could be generated, including rapid achievement of constant levels, or step-wise increased levels. The resulting tissue curves had low deviation from the target curves according to the specified criteria: AUC (%): 4.2 +/- 2.8, Maximal deviation (%): 13.6 +/- 5.0 and R2: 0.95 +/- 0.02.CONCLUSION: The UIPump-system is suitable for use in PET-research for assessment of PK/PD properties by simulation of different tracer tissue kinetics in vivo.