A third sea urchin sperm receptor for egg jelly module protein, suREJ2, concentrates in the plasma membrane over the sperm mitochondrion.
A third sea urchin sperm receptor for egg jelly module protein, suREJ2, concentrates in the plasma membrane over the sperm mitochondrion.
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海胆精子的卵果冻模块蛋白的第三个受体 suREJ2 集中在精子线粒体上方的质膜中。
DOI：
10.1111/j.1440-169x.2004.00729.x
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Vacquier,VictorD
中科院分区：
文献类型：
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作者：
Galindo,BlancaE;Moy,GaryW;Vacquier,VictorD
海胆精子是研究鞭毛运动和顶体反应的信号转导事件的模型细胞。我们先前描述了海胆精子卵冻受体1（suREJ 1），其由1450个氨基酸组成，具有一个跨膜区段，并结合到卵冻的岩藻糖硫酸酯聚合物以诱导精子顶体反应。我们还克隆了suREJ 3，它由2681个氨基酸组成，有11个推定的跨膜片段。这两种蛋白质定位于顶体囊泡上的质膜。在克隆suREJ 1的过程中，我们发现suREJ 2由1472个氨基酸组成，具有两个跨膜片段，存在于整个精子质膜中，但集中在精子膜上。实验证据表明，与suREJ 1和suREJ 3不同，suREJ 2不从质膜伸出细胞外，而是细胞内质膜蛋白。所有三种海胆精子REJ蛋白都具有一个> 900个氨基酸的蛋白质模块，称为"REJ模块“，这是由人类常染色体显性多囊肾病蛋白、多囊蛋白-1和PKDREJ共享的，PKDREJ是哺乳动物中的睾丸特异性蛋白，其功能未知。 在本研究中，我们描述了suREJ 2的序列，结构域和定位，并推测其可能的功能。
Sea urchin spermatozoa are model cells for studying signal transduction events underlying flagellar motility and the acrosome reaction. We previously described the sea urchin sperm receptor for egg jelly 1 (suREJ1) which consists of 1450 amino acids, has one transmembrane segment and binds to the fucose sulfate polymer of egg jelly to induce the sperm acrosome reaction. We also cloned suREJ3 which consists of 2681 amino acids and has 11 putative transmembrane segments. Both these proteins localize to the plasma membrane over the acrosomal vesicle. While cloning suREJ1, we found suREJ2, which consists of 1472 amino acids, has two transmembrane segments and is present in the entire sperm plasma membrane, but is concentrated over the sperm mitochondrion. Experimental evidence suggests that, unlike suREJ1 and suREJ3, suREJ2 does not project extracellularly from the plasma membrane, but is an intracellular plasma membrane protein. All three sea urchin sperm REJ proteins possess a protein module of > 900 amino acids, termed ‘the REJ module’, that is shared by the human autosomal dominant polycystic kidney disease protein, polycystin‐1, and PKDREJ, a testis‐specific protein in mammals whose function is unknown. In the present study, we describe the sequence, domain structure and localization of suREJ2 and speculate on its possible function.