Detection of a Newly Described Bacteriocin, Perfrin, Among Clostridium perfringens Isolates from Healthy and Diseased Ostriches and Broiler Chickens in Iran
Detection of a Newly Described Bacteriocin, Perfrin, Among Clostridium perfringens Isolates from Healthy and Diseased Ostriches and Broiler Chickens in Iran
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在从伊朗健康和患病鸵鸟和肉鸡中分离的产气荚膜梭菌中检测到一种新描述的细菌素（Perfrin）
DOI：
10.1637/11580-010517-resnoter
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发表时间：
2017
期刊：
影响因子：
1.4
通讯作者：
A. Zamani
中科院分区：
文献类型：
--
作者：
Jamshid Razmyar;S. M. Peighambari;A. Zamani
由携带netB基因的产气荚膜梭菌菌株引起的坏死性肠炎是家禽中的一种众所周知的疾病。本研究的目的是调查一种新的细菌素，perfrin，与netB之间的健康和患病鸵鸟和肉鸡分离株的关联。四十六度本研究包括2010年至2014年收集的来自肉鸡和鸵鸟的产气荚膜杆菌分离株，并对其进行PCR以检测netB和产气荚膜蛋白基因。在肉鸡和鸵鸟中分别有6株（60%）和9株（25%）同时检测到netB和perfrin基因。统计分析发现，健康和患病鸡群之间的perfrin在肉鸡和鸵鸟中存在显着差异。对于净B，仅在健康和患病鸵鸟群之间存在显著差异。这是首次报道在netB阳性的C.鸵鸟产气荚膜杆菌菌株。
SUMMARY Necrotic enteritis due to Clostridium perfringens strains harboring the netB gene is a well-known disorder in poultry. The aim of this study was to investigate the association of a novel bacteriocin, perfrin, with netB among isolates from healthy and diseased ostriches and broiler chickens. Forty-six C. perfringens isolates from broiler chickens and ostriches collected from 2010 to 2014 were included in this study and subjected to PCR to detect netB and perfrin genes. Six (60%) and 9 (25%) isolates were positive for both netB and perfrin genes in broilers and ostriches, respectively. Statistical analysis found a significant difference between healthy and diseased flocks for perfrin both in broilers and ostriches. For netB, the significant difference was only found between healthy and diseased ostrich flocks. This is the first report of the presence of perfrin in netB-positive C. perfringens strains in ostriches.