A Single Nucleotide Polymorphism within the Interferon Gamma Receptor 2 Gene Perfectly Coincides with Polledness in Holstein Cattle
A Single Nucleotide Polymorphism within the Interferon Gamma Receptor 2 Gene Perfectly Coincides with Polledness in Holstein Cattle
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DOI：
10.1371/journal.pone.0067992
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发表时间：
2013-06-21
期刊：
影响因子：
3.7
通讯作者：
Distl, Ottmar
中科院分区：
文献类型：
--
作者：
Glatzer, Sabrina;Merten, Nina Johanna;Distl, Ottmar
授粉是现代牛奶和牛肉生产中影响较大的性状，以满足动物福利和生产安全的要求。先前的研究已将该轮选位点定位到牛1号染色体（BTA1）的近端区域，并将其缩小到约1 Mb。对1 Mb轮选区域内的候选基因进行测序和对荷斯坦牛的全基因组测序发现，在干扰素γ受体2基因（IFNGR2）的内含子3内存在单核苷酸多态性（SNP） AC000158: g. 1390292G> a，与荷斯坦牛的轮选性完全共分离。在443只同一品种的动物身上证实了这种完全的联系。这个SNP允许可靠的角荷斯坦、杂合和纯合投票荷斯坦的基因分型，即使在动物不能解决使用先前发表的荷斯坦单倍型。
Polledness is a high impact trait in modern milk and beef production to meet the demands of animal welfare and work safety. Previous studies have mapped the polled-locus to the proximal region of the bovine chromosome 1 (BTA1) and narrowed it down to approximately 1 Mb. Sequencing of the positional candidate genes within the 1 Mb polled region and whole genome sequencing of Holsteins revealed a single nucleotide polymorphism (SNP) AC000158: g. 1390292G>A within intron 3 of the interferon gamma receptor 2 gene (IFNGR2) in perfect co-segregation with polledness in Holsteins. This complete association was validated in 443 animals of the same breed. This SNP allows reliable genotyping of horned, heterozygous and homozygous polled Holsteins, even in animals that could not be resolved using the previously published haplotype for Holstein.