The protective effect of schisandra lignans on stress-evoked hepatic metastases of P815 tumor cells in restraint mice
The protective effect of schisandra lignans on stress-evoked hepatic metastases of P815 tumor cells in restraint mice
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DOI：
10.1016/j.jep.2010.11.070
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发表时间：
2011-03-08
影响因子：
5.4
通讯作者：
Kurihara, Hiroshi
中科院分区：
文献类型：
--
作者：
Tang, Shu-Hong;He, Rong-Rong;Kurihara, Hiroshi
研究目的：探讨五味子木脂素提取物（SLE）对肥大细胞瘤P815肿瘤细胞应激诱发肝转移的影响，其与免疫功能密切相关。材料与方法：记录SLE的高效液相色谱（HPLC）指纹图谱，测定五味子木脂素的百分组成为 82.63%。研究了 SLE 的免疫调节特性对应激诱导的肝转移的保护作用的贡献。结果：我们的结果发现，约束应激显着促进 P815 肿瘤细胞的肝转移。然而，口服SLE（100和200 mg/kg/d，14天）显着减少了受限制小鼠肝脏中转移集落的数量。进一步发现SLE可显着改善应激小鼠免疫脾细胞的T淋巴细胞比例并增加细胞毒性T淋巴细胞（CTL）活性。结论：这些结果表明SLE对肝转移的保护作用与其减轻应激源对生物稳态和免疫保护的不利影响有关。获得的数据为阐明五味子作为补品或镇静剂的传统用途提供了证据。 (C) 2010 Elsevier Ireland Ltd. 保留所有权利。
Aim of the study: The present study was conducted to investigate the effects of schisandra lignans extract (SLE) on stress-evoked hepatic metastases of mastocytoma P815 tumor cells, which was closely related with immune function.Materials and methods: The high-performance liquid chromatography (HPLC) fingerprint of SLE was recorded and the percentage composition of schisandra lignans was determined as 82.63%. The contributions of the immunomodulatory properties of SLE to the protective effects on stress-induced hepatic metastases were studied.Results: Our results found that restraint stress significantly promoted hepatic metastases of P815 tumor cells. However, oral administration of SLE (100 and 200 mg/kg/d, 14d) significantly reduced the number of metastatic colonies in liver of restrained mice. SLE was further found to be significantly improving T lymphocyte proportions and increasing cytotoxic T lymphocyte (CTL) activity of immunized spleen cells in stressed mice.Conclusion: These results indicated that the protective effects of SLE on hepatic metastases were related to its alleviation of the adverse effects of stressors for bio-homeostasis and immunoprotection. The obtained data provided evidences to elucidate the traditional use of Fructus schisandrae as a tonic or sedative. (C) 2010 Elsevier Ireland Ltd. All rights reserved.