Overexpression of jumu induces melanotic nodules by activating Toll signaling in Drosophila
Overexpression of jumu induces melanotic nodules by activating Toll signaling in Drosophila
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Jumu 的过表达通过激活果蝇中的 Toll 信号诱导黑色素结节
DOI：
10.1016/j.ibmb.2016.08.002
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发表时间：
2016-10-01
影响因子：
3.8
通讯作者：
Jin, Li Hua
中科院分区：
文献类型：
--
作者：
Zhang, Gaoqun;Hao, Yangguang;Jin, Li Hua
黑色素性结节通常被认为是由异常的免疫反应引起的。几种造血突变和信号通路，包括Toll、JAK/STAT、RAS和JNK通路，当在血细胞中被特异性激活时，可以导致黑色素性结节的形成。在这里，我们使用UAS-Gal4系统在苍蝇免疫反应系统中过表达JUNEAUX(JUNU)。Jumeaux(Jumu)是Winged-Helix/Forkhead(WH/FKH)基因转录因子家族的新成员，在果蝇的生长和形态发生中起重要作用，并参与血细胞的增殖和分化。在血细胞和脂肪体中过表达的JUMU在幼虫和成虫体内产生了许多黑色素性结节。在脂肪体内观察到的结节被大量的血细胞包围，其过程看起来类似于异物包裹。这种现象是由Toll通路激活引起的，导致血细胞沉积在脂肪体内。此外，我们还报道了脂肪细胞的解离和血细胞的异常增殖和分化。这些结果提示Jumu介导的造血和脂肪体之间的串扰，特别是在Toll依赖的黑色素性结节的形成过程中。(C)2016爱思唯尔有限公司。保留所有权利。
Melanotic nodules are commonly assumed to be caused by an abnormal immune response. Several hematopoietic mutants and signaling pathways, including the Toll, JAK/STAT, Ras and JNK pathways, can cause melanotic nodules to develop when specifically activated in hemocytes. Here, we used the UAS-Gal4 system to overexpress jumeaux (jumu) in the fly immune response system. Jumeaux (Jumu) is a new member of the winged-helix/forkhead (WH/FKH) gene family of transcription factors, which plays an important role in the growth and morphogenesis of Drosophila and participates in the proliferation and differentiation of hemocytes. Overexpressing jumu in both hemocytes and the fat body generated many melanotic nodules in larvae and adult flies. The nodules observed in the fat body were surrounded by large numbers of blood cells through a process that appeared similar to foreign body encapsulation. This phenomenon is caused by Toll pathway activation and leads to blood cells deposited in the fat body. In addition, we also report the dissociation of fat cells and the abnormal proliferation and differentiation of blood cells. These results suggest a Jumu-mediated crosstalk between hematopoiesis and the fat body, especially during the Toll-dependent formation of melanotic nodules. (C) 2016 Elsevier Ltd. All rights reserved.