Measurement of IgE on human basophils: relation to serum IgE and anti-IgE-induced histamine release.
Measurement of IgE on human basophils: relation to serum IgE and anti-IgE-induced histamine release.
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人嗜碱性粒细胞 IgE 的测量：与血清 IgE 和抗 IgE 诱导的组胺释放的关系。
DOI：
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发表时间：
1977
影响因子：
4.4
通讯作者：
L. Lichtenstein
中科院分区：
文献类型：
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作者：
M. C. Conroy;N. Adkinson;L. Lichtenstein
结合到人嗜碱性粒细胞的IgE分子的数量是通过直接测量将白细胞暴露于冷的pH 3.7乙酸盐缓冲液后解离的IgE来计算的。在研究的18名供体中，细胞结合IgE的范围为4000至500，000分子/嗜碱性粒细胞，并与血清IgE浓度（5至3，000 ng/ml）相关（r = 0.89，p <0.001）。测试这些细胞对抗IgE的敏感性以探索细胞结合的IgE与组胺释放所需的抗IgE浓度之间的关系。来自一些非特应性供体的细胞（4000至100，000 IgE分子/嗜碱性粒细胞）与来自过敏性供体的细胞（100，00至500，000 IgE分子/嗜碱性粒细胞）一样敏感。此外，来自具有大致相同的细胞结合IgE浓度的供体的细胞在其对抗IgE的敏感性方面变化很大。我们的结论是，人类嗜碱性粒细胞的内在属性（“可释放性”）是一个重要的参数，在确定介质释放。
The number of IgE molecules bound to human basophils was calculated from direct measurements of the IgE dissociated after exposing leukocytes to pH 3.7 acetate buffer in the cold. In 18 donors studied, cell-bound IgE ranged from 4000 to 500,000 molecules/basophil and correlated with the serum IgE concentration (r = 0.89, p less than 0.001) which ranged from 5 to 3,000 ng/ml. Sensitivity of these cells to anti-IgE was tested to explore the relationship between cell-bound IgE and the concentration of anti-IgE required for histamine release. Cells from some nonatopic donors (4000 to 100,000 IgE molecules/basophil) were as sensitive as cells from allergic donors (100,00 to 500,000 IgE molecules/basophil). Moreover, cells from donors having approximately the same cell-bound IgE concentration varied widely in their sensitivity to anti-IgE. We conclude that an intrinsic property of human basophils ("releasability") is an important parameter in determing mediator release.