Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms
Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms
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DOI：
10.1364/ao.46.000993
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发表时间：
2007-02-20
期刊：
影响因子：
1.9
通讯作者：
Brooker, Gary
中科院分区：
文献类型：
--
作者：
Indebetouw, Guy;Tada, Yoshitaka;Brooker, Gary
我们提出了我们认为是一个新的应用扫描全息显微镜超分辨率。通过对几幅离轴菲涅耳全息图的再现进行数字相干叠加，获得了超过物镜瑞利极限的空间分辨率。简要回顾了全息叠加和合成孔径超分辨技术的发展历史。该方法通过结合三个离轴全息图的荧光珠显示了近2倍的横向分辨率增益的实验证明。
We present what we believe to be a new application of scanning holographic microscopy to superresolution. Spatial resolution exceeding the Rayleigh limit of the objective is obtained by digital coherent addition of the reconstructions of several off-axis Fresnel holograms. Superresolution by holographic superposition and synthetic aperture has a long history, which is briefly reviewed. The method is demonstrated experimentally by combining three off-axis holograms of fluorescent beads showing a transverse resolution gain of nearly a factor of 2.