ATP decreases acutely and reversibly transport through the paracellular pathway in human cervical cells.
ATP decreases acutely and reversibly transport through the paracellular pathway in human cervical cells.
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ATP 在人宫颈细胞中通过细胞旁途径急剧且可逆地运输。
DOI：
10.1152/ajpcell.1994.266.6.c1692
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Romero,MF
中科院分区：
文献类型：
--
作者：
Gorodeski,GI;Hopfer,U;Eckert,RL;Utian,WH;DeSantis,BJ;Rorke,EA;Romero,MF
我们研究了三磷酸腺苷对人宫颈细胞旁细胞跨上皮转运的影响。在生长在可渗透载体上的Caski细胞中，测量了跨上皮电导和跨上皮通透性(通过测量惰性分子的单向流量来确定)。0.51 kDa探头跨血管电导为55.9±17.7ms/cm2，通透性为12.5+/-2.7x10(-6)cm/S。加入ATP后，0.18~10 kDa探针的电导和通透性显著降低23~31%，且呈剂量依赖性，半数有效浓度为1微米，在胞外5~10微米时作用最大。无论上皮细胞的压力梯度如何，都能观察到ATP效应。这些结果表明，细胞外微摩尔浓度的三磷酸腺苷可通过影响紧密连接的通透性和细胞间隙的阻力而显著且可逆地降低宫颈细胞旁通路的通透性。在这些数据的基础上，我们推测，在活体内，ATP可能在调节溶质和液体通过宫颈上皮的运输中发挥作用。
We studied the effect of ATP on transepithelial transport through the paracellular pathway in human cervical cells. Transepithelial conductance and transepithelial permeability (determined from the measurements of unidirectional flux of inert molecules) were measured in Caski cells grown on permeable support. Transepithelial conductance was 55.9 +/- 17.7 mS/cm2 and permeability was 12.5 +/- 2.7 x 10(-6) cm/s for a 0.51-kDa probe. Addition of ATP to the medium decreased acutely and reversibly the conductance and the permeability to probes between 0.18 and 10 kDa by 23-31% in a dose-related fashion; the 50% effective concentration was 1 microM, with a maximal effect at 5-10 microM extracellular ATP. The ATP effect was observed regardless of the pressure gradient across the epithelium. These results indicate that extracellular ATP in micromolar concentrations decreases acutely and reversibly the permeability through the paracellular pathway in cervical cells, possibly by affecting the permeability of the tight junctions and the resistance of the intercellular space. On the basis of these data, we speculate that ATP may play a role in the regulation of solutes and fluid transport across the cervical epithelium in vivo.