2′-Fluoroarabinonucleic Acid Nanostructures as Stable Carriers for Cellular Delivery in the Strongly Acidic Environment
2′-Fluoroarabinonucleic Acid Nanostructures as Stable Carriers for Cellular Delivery in the Strongly Acidic Environment
复制标题
2-氟阿拉伯核酸纳米结构作为强酸性环境中细胞传递的稳定载体
DOI：
10.1021/acsami.0c11684
复制
发表时间：
2020
影响因子：
9.5
通讯作者：
Li Z.
中科院分区：
文献类型：
--
作者：
Wang Q.;Chen X.;Li X.;Song D.;Yang J.;Yu H.;Li Z.
DNA 纳米技术在构建具有不同尺寸、大小和形状的可编程纳米结构方面具有强大的功能。然而，天然DNA分子容易被核酸酶降解，从而限制了这种DNA纳米结构的体内应用。 2'-氟阿拉伯核酸 (FANA) 是一种化学修饰的寡核苷酸，具有与 DNA 相似的碱基配对特性，并表现出优异的物理和化学稳定性。在这项工作中，FANA分子被用来构建双交换纳米结构，并证明FANA的掺入赋予核酸纳米结构更高的热稳定性和更强的核酸酶抗性。更重要的是，FANA纳米结构能够在强酸性环境（pH 1.2）中保持结构完整性。最后，这种 FANA 纳米结构在模拟胃液中作为细胞递送小分子货物的稳定载体方面发挥了良好的作用，而 DNA 对应物大部分被降解。总的来说，这些结果表明 FANA 自组装不仅是结构 DNA 纳米技术的实质性补充，而且还是体内生物医学应用的一个有吸引力的分子工具。
DNA nanotechnology is powerful in constructing programmable nanostructures with distinct dimensions, sizes, and shapes. However, natural DNA molecules are prone to nuclease degradation, thus limiting thein vivoapplications of such DNA nanostructures. 2′-Fluoroarabinonucleic acid (FANA) is a chemically modified oligonucleotide with similar base pairing properties to DNA and exhibits superior physical and chemical stabilities. In this work, FANA molecules were used to construct double crossover nanostructures, and it was demonstrated that incorporation of FANA conferred nucleic acid nanostructures with increased thermal stability and stronger nuclease resistance. More importantly, FANA nanostructures were able to maintain the structural integrity in the strongly acidic environment (pH 1.2). Last, such FANA nanostructures functioned well in acting as stable carriers of small-molecule cargoes for cellular delivery in simulated gastric fluid, while the DNA counterparts were mostly degraded. Collectively, these results demonstrated that FANA self-assembly was not only a substantial complement to the structural DNA nanotechnology but also an appealing molecular tool forin vivobiomedical applications.