Detection of nuclear lamin B epitopes in oocyte nuclei from mice, sea urchins, and clams using a human autoimmune serum.
Detection of nuclear lamin B epitopes in oocyte nuclei from mice, sea urchins, and clams using a human autoimmune serum.
复制标题
使用人自身免疫血清检测小鼠、海胆和蛤的卵母细胞核中的核纤层蛋白 B 表位。
DOI：
10.1016/0012-1606(87)90173-4
复制
发表时间：
1987
影响因子：
2.7
通讯作者：
Carrera,AE
中科院分区：
文献类型：
--
作者：
Maul,GG;Schatten,G;Jimenez,SA;Carrera,AE
体细胞核通常含有两种或三种主要蛋白质，核纤层蛋白A、B和C或其抗原相关的等同物，散布在染色质及其附着位点，内核膜之间。然而，先前研究过的Xenopus和Spisulageresophagus囊泡的晚期卵母细胞核膜没有染色质附着，只有一个层粘连蛋白样蛋白。由于小鼠和海胆的生殖囊泡有染色质附着，我们测试了它们是否可能存在一个以上的核纤层蛋白。在这两个物种中，我们发现两个不同的核纤层蛋白纳入其核膜结构。一个核纤层蛋白被抗核纤层蛋白B识别，另一个被抗核纤层蛋白AC抗体识别。发现螺旋囊泡不仅含有核纤层蛋白（核纤层蛋白），而且还含有与抗核纤层蛋白B抗体交叉反应的65-kDa蛋白。这种蛋白质不附着于任何结构，显然是可溶的。我们的研究结果提供了一个可能的解释的早期存在的核纤层蛋白B在小鼠和海胆的原核相反的晚出现的核纤层蛋白B相当于两栖动物胚胎。在Spisula，在非洲爪蟾，核纤层蛋白B等价物的存在下，不能记录在早期胚胎的核膜，表明一个单独的核纤层蛋白B等价物是不必要的染色质结合到这些物种的包膜在早期胚胎发生。这些结果还表明，在不同物种的同一细胞类型中，结构蛋白的核补体可能有很大差异。
Somatic nuclei typically contain two or three major proteins, the lamins A, B, and C or their antigenically related equivalents, interspersed between the chromatin and its attachment site, the inner nuclear membrane. The late oocyte nuclear envelopes of the previously investigatedXenopusandSpisulagerminal vesicles, however, have no chromatin attached and only one lamin-like protein. Since mouse and sea urchin germinal vesicles have chromatin attached, we tested them for the possible presence of more than one lamin. In both species we found two different lamins incorporated in their nuclear envelope structure. One lamin is recognized by anti-lamin B and the other by anti-lamin AC antibodies.Spisulagerminal vesicles were found to contain not only the nuclear envelope-bound lamin (clamin), but also a 65-kDa protein cross-reactive with anti-lamin B antibodies. This protein is present unattached to any structure and is apparently soluble. Our findings provide a possible explanation of the early presence of lamin B in pronuclei of mouse and sea urchin contrary to the late appearance of a lamin B equivalent in amphibian embryos. InSpisula, as inXenopus, the presence of a lamin B equivalent could not be documented in the nuclear envelopes of early embryos, indicating that a separate lamin B equivalent is not essential for chromatin binding to the envelope in these species during early embryogenesis. The results also indicate that the nuclear complement of structural proteins might vary substantially in the same cell type of different species.