Antioxidant properties of crocin from Gardenia jasminoides Ellis and study of the reactions of crocin with linoleic acid and crocin with oxygen
Antioxidant properties of crocin from Gardenia jasminoides Ellis and study of the reactions of crocin with linoleic acid and crocin with oxygen
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DOI：
10.1021/jf991263j
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发表时间：
2000-05-01
影响因子：
6.1
通讯作者：
Van Calsteren, MR
中科院分区：
文献类型：
--
作者：
Pham, TQ;Cormier, F;Van Calsteren, MR
藏红花素是一种水溶性类胡萝卜素，存在于栀子花（栀子花）和藏红花（藏红花）的柱头中。栀子苷的纯化采用50%丙酮提取栀子，乙醚洗涤，离子交换，制备高效液相色谱分离。纯度为>99.6%的纯化藏红花素在浓度高达40ppm时具有抗氧化活性。在20ppm时，藏红花素的抗氧化活性与BHA相当。硫氰酸法测定藏红花素的抗氧化性能优于硫代巴比妥酸法。用液相色谱-质谱法测定了亚油酸自由基与藏红花素之间的加合物。当藏红花素在FeSO4存在下与氧反应时，形成藏红花素的单氢过氧化物和双氢过氧化物等中间体，并通过LC-MS检测。
Crocin-a water soluble carotenoid-is found in the fruits of gardenia (Gardenia jasminoides Ellis) and in the stigmas of saffron (Crocus sativus Linne). For crocin purification, gardenia fruits are extracted with 50% acetone, followed by ether washing, ion exchange, and separation by preparative HPLC. Purified crocin with purity of >99.6% has an antioxidative activity at concentrations up to 40 ppm. At 20 ppm the antioxidative activity of crocin is comparable to that of BHA. The antioxidant property of crocin as evaluated by the thiocyanate method was better than with the thiobarbituric acid method. The adduct between the linoleic acid radical and crocin was detected by LC-MS. When crocin reacted with oxygen in the presence of FeSO4, intermediates such as monohydroperoxides and dihydroperoxides of crocin were formed and detected by LC-MS.