Low concentrations of NaHSO3 enhance NAD(P)H dehydrogenase-dependent cyclic photophosphorylation and alleviate the oxidative damage to improve photosynthesis in tobacco
Low concentrations of NaHSO3 enhance NAD(P)H dehydrogenase-dependent cyclic photophosphorylation and alleviate the oxidative damage to improve photosynthesis in tobacco
复制标题
低浓度的 NaHSO3 增强 NAD(P)H 脱氢酶依赖性循环光磷酸化并减轻氧化损伤，从而改善烟草的光合作用
DOI：
10.1007/s11434-012-5309-6
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发表时间：
2012
影响因子：
--
通讯作者：
H. Mi
中科院分区：
文献类型：
--
作者：
Yanxia Wu;Weiming He;Weimin Ma;Yunkang Shen;H. Mi
低浓度的亚硫酸氢盐（L-NaHSO 3）增加了光系统I（PSI）周围的循环电子传递和光合作用。然而，鲜为人知的是，关于详细的贡献循环电子传递促进光合作用的L-NaHSO 3。本文以烟草突变体indhC-ndhK-ndhJ（ΔndhCKJ）为材料，研究了NAD（P）H脱氢酶（NDH）依赖的PSI周围的电子传递在L-NaHSO 3提高光合作用中的作用。用L-NaHSO 3（10 μmol L−1）处理烟草叶片后，通过Chl荧光和NDH量的瞬时照射后增加监测的NDH依赖的循环电子传递在野生型（WT）中显著上调。Δ ndhCKJ细胞依赖于NDH的循环电子传递受到严重损害，而L-NaHSO 3处理对该过程无明显影响。相应地，L-NaHSO 3增加了WT中依赖于NDH的类囊体膜质子梯度（ΔpH）（毫秒延迟发光（ms-DLE）的慢相），但对ΔndhCKJ没有影响; L-NaHSO 3对WT中PSP活性的增强作用比ΔndhCKJ更明显。WT经L-NaHSO 3处理后，超氧阴离子和过氧化氢的积累均减少，而ΔndhCKJ则无此变化。此外，L-NaHSO 3对WT叶片光合放氧速率的促进作用大于ΔndhCKJ。因此，我们得出结论，L-NaHSO 3通过增强NDH依赖的环状PSP来减轻光氧化损伤，从而提高光合作用。
Bisulfite at low concentrations (L-NaHSO3) increases cyclic electron transport around photosystem I (PSI) and photosynthesis. However, little is known regarding the detailed contribution of cyclic electron transport to the promoted photosynthesis by L-NaHSO3. In the present work, we used tobacco mutant defective inndhC-ndhK-ndhJ(ΔndhCKJ) to investigate the role of NAD(P)H dehydrogenase (NDH)-dependent cyclic electron transport around PSI in an increase in photosynthesis by L-NaHSO3. After the treatment of tobacco leaves with L-NaHSO3(10 μmol L−1), the NDH-dependent cyclic electron transport, monitored by a transient post-illumination increase in Chl fluorescence and the amount of NDH, was notably up-regulated in wild type (WT). The NDH-dependent cyclic electron transport was severely impaired inΔndhCKJand was not significantly affected by treatment with L-NaHSO3. Accordingly, the NDH-dependent transthylakoid membrane proton gradient (ΔpH), as reflected by the slow phase of millisecond-delayed light emission (ms-DLE), was increased by L-NaHSO3in WT, but not in ΔndhCKJ; the enhancement of cyclic photophosphorylation (PSP) activity by L-NaHSO3was more obvious in WT than ΔndhCKJ. The accumulation of both superoxide and hydrogen peroxide was reduced in WT when subjected to L-NaHSO3treatment, but not in ΔndhCKJ. Furthermore, the increase of photosynthetic O2evolution rate by L-NaHSO3was more significant in WT than in ΔndhCKJ. We therefore conclude that L-NaHSO3alleviates the photo-oxidative damage by the enhancement of NDH-dependent cyclic PSP, thereby improving photosynthesis.
影响因子：
4.3
作者：
Ishida, H;Shimizu, S;Mae, T
通讯作者：
Mae, T
影响因子：
7.4
作者：
Asada, Kozi
通讯作者：
Asada, Kozi