ZmMKK4, a novel group C mitogen-activated protein kinase kinase in maize (Zea mays), confers salt and cold tolerance in transgenic Arabidopsis
ZmMKK4, a novel group C mitogen-activated protein kinase kinase in maize (Zea mays), confers salt and cold tolerance in transgenic Arabidopsis
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ZmMKK4 是玉米 (Zea mays) 中一种新型 C 族丝裂原激活蛋白激酶激酶，赋予转基因拟南芥耐盐和耐冷能力
DOI：
10.1111/j.1365-3040.2011.02329.x
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发表时间：
2011-08-01
影响因子：
7.3
通讯作者：
Li, Dequan
中科院分区：
文献类型：
--
作者：
Kong, Xiangpei;Pan, Jiaowen;Li, Dequan
丝裂原活化蛋白激酶（MAPK）级联是将细胞外信号传导到一系列细胞反应的信号传导模块。植物MAPK级联反应与发育和胁迫反应有关。在这项研究中，我们从玉米中分离到了一个新的C组MAPKK基因ZmMKK4。Northern blotting分析显示，低温、高盐和外源H2O2上调ZmMKK4转录本的表达，而外源ABA下调ZmMKK4转录本的表达。与对照植株相比，ZmMKK4的过表达提高了拟南芥的发芽率、侧根数、植株存活率、叶绿素、脯氨酸和可溶性糖含量以及抗氧化酶[过氧化物酶（POD）、过氧化氢酶（CAT）]活性，从而提高了拟南芥对寒冷和盐胁迫的耐受性。此外，ZmMKK4在低温和盐胁迫下增强了37 kDa激酶活性。RT-PCR分析显示，zmmkk4过表达植株中胁迫应答转录因子和功能基因的转录水平高于对照植株。此外，ZmMKK4蛋白定位于细胞核。综上所述，这些结果表明ZmMKK4是植物耐盐性和耐寒性的正调节因子。
Mitogen-activated protein kinase (MAPK) cascades are signalling modules that transduce extracellular signalling to a range of cellular responses. Plant MAPK cascades have been implicated in development and stress response. In this study, we isolated a novel group C MAPKK gene, ZmMKK4, from maize. Northern blotting analysis revealed that the ZmMKK4 transcript expression was up-regulated by cold, high salt and exogenous H2O2, but down-regulated by exogenous abscisic acid (ABA). Over-expression of ZmMKK4 in Arabidopsis conferred tolerance to cold and salt stresses by increased germination rate, lateral root numbers, plant survival rate, chlorophyll, proline and soluble sugar contents, and antioxidant enzyme [peroxidase (POD), catalase (CAT)] activities compared with control plants. Furthermore, ZmMKK4 enhanced a 37 kDa kinase activity after cold and salt stresses. RT-PCR analysis revealed that the transcript levels of stress-responsive transcription factors and functional genes were higher in ZmMKK4-over-expressing plants than in control plants. In addition, ZmMKK4 protein is localized in the nucleus. Taken together, these results indicate that ZmMKK4 is a positive regulator of salt and cold tolerance in plants.