Increased cerebral (R)-[(11)C]PK11195 uptake and glutamate release in a rat model of traumatic brain injury: a longitudinal pilot study.
Increased cerebral (R)-[(11)C]PK11195 uptake and glutamate release in a rat model of traumatic brain injury: a longitudinal pilot study.
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DOI：
10.1186/1742-2094-8-67
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发表时间：
2011-06-14
影响因子：
9.3
通讯作者：
Molthoff CF
中科院分区：
文献类型：
--
作者：
Folkersma H;Foster Dingley JC;van Berckel BN;Rozemuller A;Boellaard R;Huisman MC;Lammertsma AA;Vandertop WP;Molthoff CF
本研究的目的是调查小胶质细胞激活随着时间的推移创伤性脑损伤（TBI），并将这些发现与谷氨酸释放。在TBI前24小时（基线）和TBI后1天和10天，使用受控皮质撞击或假手术对大鼠进行连续动态（R）-[11 C] PK 11195 PET扫描。细胞外液（ECF）谷氨酸浓度测定脑微透析。对脑进行组织病理学和（免疫）组织化学处理。TBI后10天，与基线值和假手术对照相比，TBI大鼠中的（R）-[11 C] PK 11195结合显著增加（p < 0.05）。脑损伤后即刻ECF谷氨酸值（27.6 ± 14.0 μmol·L-1）较假手术组（6.4 ± 3.6 μmol·L-1）明显升高。在TBI和假手术之间发现艾德-1、OX-6、GFAP、Perl's和Fluoro-Jade B的显著差异。脑外伤后10天（R）-[11 C] PK 11195的脑摄取增加表明小胶质细胞的长期和持续活化。这种激活后，ECF谷氨酸水平的显着急性创伤后增加。
The aim of the present study was to investigate microglia activation over time following traumatic brain injury (TBI) and to relate these findings to glutamate release. Sequential dynamic (R)-[11C]PK11195 PET scans were performed in rats 24 hours before (baseline), and one and ten days after TBI using controlled cortical impact, or a sham procedure. Extracellular fluid (ECF) glutamate concentrations were measured using cerebral microdialysis. Brains were processed for histopathology and (immuno)-histochemistry. Ten days after TBI, (R)-[11C]PK11195 binding was significantly increased in TBI rats compared with both baseline values and sham controls (p < 0.05). ECF glutamate values were increased immediately after TBI (27.6 ± 14.0 μmol·L-1) as compared with the sham procedure (6.4 ± 3.6 μmol·L-1). Significant differences were found between TBI and sham for ED-1, OX-6, GFAP, Perl's, and Fluoro-Jade B. Increased cerebral uptake of (R)-[11C]PK11195 ten days after TBI points to prolonged and ongoing activation of microglia. This activation followed a significant acute posttraumatic increase in ECF glutamate levels.
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影响因子：
3.9
作者：
Hardingham GE
通讯作者：
Hardingham GE
影响因子：
4.1
作者：
Bullock, R;Zauner, A;Young, HF
通讯作者：
Young, HF
影响因子：
6.3
作者：
Kropholler, Marc A.;Boellaard, Ronald;Lammertsma, Adriaan A.
通讯作者：
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影响因子：
3.5
作者：
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通讯作者：
Lammertsma, Adriaan A.
影响因子：
1.9
作者：
Suma, Takeshi;Koshinaga, Morimichi;Katayama, Yoichi
通讯作者：
Katayama, Yoichi