Comparative Analysis of Bacterial and Archaeal Communities in Methanogenic Sludge Granules from Upflow Anaerobic Sludge Blanket Reactors Treating Various Food-Processing, High-Strength Organic Wastewaters
Comparative Analysis of Bacterial and Archaeal Communities in Methanogenic Sludge Granules from Upflow Anaerobic Sludge Blanket Reactors Treating Various Food-Processing, High-Strength Organic Wastewaters
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DOI：
10.1264/jsme2.me08561
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发表时间：
2009-01-01
影响因子：
2.2
通讯作者：
Sekiguchi, Yuji
中科院分区：
文献类型：
--
作者：
Narihiro, Takashi;Terada, Takeshi;Sekiguchi, Yuji
采用16S rRNA基因克隆文库法对12种不同类型的食品废水处理升流式厌氧污泥床（UASB）反应器颗粒污泥中的细菌和古菌群落结构进行了全面调查。总共分析了1，282个细菌16S rRNA基因克隆和722个古细菌克隆，并通过系统发育分析确定了它们的身份。总体而言，在所检查的细菌克隆文库中大量观察到属于细菌门变形菌门（特别是δ变形菌门）、厚壁菌门、螺旋体门和拟杆菌门的克隆，表明在这种处理系统中常见的细菌降解剂。在该领域内，甲烷微菌和甲烷菌属的克隆被发现是丰富的古细菌库。关于反应器性能的特点（如化学需氧量去除，脂肪酸积累和污泥膨胀），可能的代表性的污泥类型可能与工艺失败，如污泥膨胀和丙酸盐的积累，被发现在污泥库中的污泥类型的分布的比较分析。
A comprehensive survey of bacterial and archaeal community structures within granular sludges taken from twelve different types of full-scale, food-processing wastewater-treating, upflow anaerobic sludge blanket (UASB) reactors was performed with a 16S rRNA gene-based clone library method. In total, 1,282 bacterial 16S rRNA gene clones and 722 archaeal clones were analyzed, and their identities were determined by phylogenetic analyses. Overall, clones belonging to the bacterial phyla Proteobacteria (the class Deltaproteobacteria in particular), Firmicutes, Spirochaetes, and Bacteroidetes were observed in abundance within the bacterial clone libraries examined, indicating common bacterial denominators in such treatment systems. Within the domain Archaea, clones affiliated with the classes Methanomicrobia and Methanobacteria were found to be abundant in the archaeal libraries. In relation to features of reactor performance (such as chemical oxygen demand removal, fatty acid accumulation, and sludge bulking), possible representative phylotypes likely to be associated with process failures, such as sludge bulking and the accumulation of propionate, were found in comparative analyses of the distribution of phylotypes in the sludge libraries.