Modulation of intestinal and systemic immune responses to a fed protein antigen, in mice.
Modulation of intestinal and systemic immune responses to a fed protein antigen, in mice.
复制标题
调节小鼠对进食蛋白抗原的肠道和全身免疫反应。
作者：
S. Strobel;A. Ferguson
给正常成年小鼠喂食蛋白质抗原会导致全身免疫低反应性（口服耐受性）。局部粘膜细胞介导的免疫通常不会被激发。这些实验的目的是消除口服耐受性的诱导，并伴随诱导小鼠局部粘膜细胞介导的免疫应答;从而确定肠道生理学或免疫学的哪些方面与诱导对喂食抗原的正常（主要是抑制性）免疫应答相关。蛋白质抗原（卵清蛋白）喂养的动物，其中免疫状态已被调制的N-乙酰胞壁酰二肽（MDP），通过诱导移植物抗宿主反应，或自然，凭借不成熟的腹腔注射。在所有给药组中均预防了经口给药耐受的诱导。在第二个系列的实验中，小鼠如前所述口服免疫，休息四周，然后在其饮用水中用卵清蛋白攻击10天。空肠结构没有改变抗原的挑战，但MDP治疗和未成熟的动物已致敏卵清蛋白，后来再次暴露于相同的抗原有显着较高的上皮内淋巴细胞计数比适当的控制。可能导致口服耐受性的消除和诱导肠道过敏的因素进行了讨论，在有关的食物过敏性疾病的人。
Feeding of a protein antigen to normal adult mice results in systemic immunologic hyporesponsiveness (oral tolerance). Local mucosal cell mediated immunity is not usually elicited. The objectives of these experiments were to abrogate the induction of oral tolerance and concomitantly to induce a local mucosal cell mediated immune response in mice; and thereby to establish which facets of intestinal physiology or immunology are relevant to the induction of normal, mainly suppressor immune responses to a fed antigen. Protein antigen (ovalbumin) was fed to animals in whom immune status had been modulated by intraperitoneal injection of N-acetyl-muramyl dipeptide (MDP), by induction of a graft versus host reaction, or naturally, by virtue of immaturity. The induction of oral tolerance was prevented in all treatment groups. In a second series of experiments mice were orally immunised as before, rested for four weeks, and then challenged with ovalbumin in their drinking water for 10 days. Jejunal architecture was not altered by the antigen challenge, but MDP treated and immature animals which had been sensitised to ovalbumin and later re-exposed to the same antigen had significantly higher intraepithelial lymphocyte counts than appropriate controls. Factors which may lead to abrogation of oral tolerance and induction of intestinal hypersensitivity are discussed in relation to food allergic diseases in man.