Gene expression profile during acute rejection in rat-to-mouse concordant cardiac xenograft by means of DNA microarray
Gene expression profile during acute rejection in rat-to-mouse concordant cardiac xenograft by means of DNA microarray
复制标题
利用 DNA 微阵列研究大鼠-小鼠一致心脏异种移植物急性排斥过程中的基因表达谱
DOI：
10.1007/s00147-002-0450-9
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发表时间：
2002
影响因子：
3.1
通讯作者：
M. Makuuchi
中科院分区：
文献类型：
--
作者：
A. Saiura;Y. Sugawara;Y. Harihara;M. Sata;T. Hamakubo;T. Kodama;M. Makuuchi
摘要：利用大鼠到小鼠的心脏移植模型和DNA芯片，我们研究了急性排斥反应期间的基因表达谱。我们用近交系BALB/c、C3H/He小鼠和Lewis大鼠进行了异位心脏移植。于移植后第5天从异种移植物(Lewis-C3 H)、同种异体移植(BALB/c-C3 H)、大鼠同系移植物(Lewis-Lewis)和小鼠同系移植物(C3-C3 H)中提取总RNA。我们通过DNA微阵列筛选异种移植物、同种异体移植物和小鼠同种移植物中的基因表达谱。通过小鼠阵列，我们确定了许多干扰素-γ诱导基因在同种移植物和异种移植物中相对于同种移植物都有深刻的表达。与同种异体移植物相比，异种移植物中特异性地诱导了Mac-1。使用大鼠阵列，我们观察到与大鼠同种移植物相比，心钠素和心钠素在异种移植物中表达最深入。除了已知的基因外，在异种移植瘤中还诱导了许多表达序列标签。我们鉴定了一组基因，包括在异种移植物中特异诱导的Mac-1，以及许多在异种移植物中上调的新基因。
Abstract.Using a rat-to-mouse concordant cardiac transplantation model and DNA microarrays, we studied the gene expression profiles during acute rejection. We used inbred BALB/c and C3H/He mice and Lewis rats for our study, in which heterotopic cardiac transplantations were performed. Total RNA was isolated from xenografts (Lewis to C3H), allografts (BALB/c to C3H), rat isografts (Lewis to Lewis) and mouse isografts (C3H to C3H) on day 5 following transplantation. We screened for gene expression profiles in the xenografts, allografts, and mouse isografts by means of DNA microarrays. With a murine array, we determined that many IFN-γ inducible genes were profoundly expressed in both the allografts and xenografts relative to the isografts. Mac-1 was specifically induced in the xenografts relative to the allografts. Using a rat array, we observed that the cardionatrin and atrial natriuretic factors were most profoundly expressed in the xenografts in comparison with the rat isografts. In addition to known genes, many expressed sequence tags were induced in the xenografts. We identified a group of genes, including Mac-1 induced specifically in xenografts, as well as many new genes upregulated in xenografts.
影响因子：
6.2
作者：
M. Blakely;W. J. van der Werf;M. Berndt;A. Dalmasso;F. Bach;W. Hancock
通讯作者：
M. Blakely;W. J. van der Werf;M. Berndt;A. Dalmasso;F. Bach;W. Hancock
影响因子：
6.2
作者：
Diamond, LE;McCurry, KR;Logan, JS
通讯作者：
Logan, JS
影响因子：
2.2
作者：
Chen, RH;Kadner, A;Adams, DH
通讯作者：
Adams, DH