Colour compound lenses for a portable fluorescence microscope
Colour compound lenses for a portable fluorescence microscope
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用于便携式荧光显微镜的彩色复合透镜
DOI：
10.1038/s41377-019-0187-1
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发表时间：
2019-08-21
影响因子：
19.4
通讯作者：
Zhang, Dawei
中科院分区：
文献类型：
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作者：
Dai, Bo;Jiao, Ziao;Zhang, Dawei
在这篇文章中，我们展示了一种手持式智能手机荧光显微镜(HSFM)，它将双功能聚合物镜头与智能手机集成在一起。HSFM由一部智能手机、一个现场便携照明源和一个同时执行光学成像和过滤的双功能聚合物透镜组成。因此，与现有的智能手机荧光显微镜相比，HSFM不需要任何额外的滤光片。尽管传统上荧光成像因其对细胞、蛋白质、DNA/RNA等的高特异性和敏感性而在生物医学和临床应用中扮演着不可或缺的角色，但传统荧光显微镜的笨重元件使其不方便用于医疗诊断。本文中演示的HSFM通过提供多功能、微型、小型化的荧光模块解决了这个问题。这种多功能荧光模块可以无缝连接到任何智能手机相机，以细胞级的分辨率进行明场和荧光成像，而无需使用额外的笨重镜头/滤镜；事实上，HSFM使用一个镜头实现放大和滤光。利用该装置进行细胞和组织观察、细胞计数、质粒转染率评价和超氧化物歧化产物分析。值得注意的是，这种镜头系统具有与众多智能手机一起工作的独特能力，无论智能手机型号和每台设备内置的摄像头技术如何。因此，这种HSFM具有为实时护理点诊断铺平道路的潜力，并为个性化医疗开辟了无数可能性。
In this article, we demonstrated a handheld smartphone fluorescence microscope (HSFM) that integrates dual-functional polymer lenses with a smartphone. The HSFM consists of a smartphone, a field-portable illumination source, and a dual-functional polymer lens that performs both optical imaging and filtering. Therefore, compared with the existing smartphone fluorescence microscope, the HSFM does not need any additional optical filters. Although fluorescence imaging has traditionally played an indispensable role in biomedical and clinical applications due to its high specificity and sensitivity for detecting cells, proteins, DNAs/RNAs, etc., the bulky elements of conventional fluorescence microscopes make them inconvenient for use in point-of-care diagnosis. The HSFM demonstrated in this article solves this problem by providing a multifunctional, miniature, small-form-factor fluorescence module. This multifunctional fluorescence module can be seamlessly attached to any smartphone camera for both bright-field and fluorescence imaging at cellular-scale resolutions without the use of additional bulky lenses/filters; in fact, the HSFM achieves magnification and light filtration using a single lens. Cell and tissue observation, cell counting, plasmid transfection evaluation, and superoxide production analysis were performed using this device. Notably, this lens system has the unique capability of functioning with numerous smartphones, irrespective of the smartphone model and the camera technology housed within each device. As such, this HSFM has the potential to pave the way for real-time point-of-care diagnosis and opens up countless possibilities for personalized medicine.