Hypoxia appears at pre-arthritic stage and shows co-localization with early synovial inflammation in collagen induced arthritis.
Hypoxia appears at pre-arthritic stage and shows co-localization with early synovial inflammation in collagen induced arthritis.
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发表时间：
2008-07
影响因子：
3.7
通讯作者：
C. Jeon;J. Ahn;Chai Jy;Kim Hj;Bae Ek;S. Park;Chow Ey;H. Cha;Ahn Ks;Koh Em
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文献类型：
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作者：
C. Jeon;J. Ahn;Chai Jy;Kim Hj;Bae Ek;S. Park;Chow Ey;H. Cha;Ahn Ks;Koh Em
目的类风湿性关节炎滑膜组织中存在缺氧现象，但缺氧与滑膜炎的关系尚不清楚。本研究旨在探讨关节炎前或炎症性关节病早期缺氧与滑膜炎的时空关系及严重程度的相关性。方法DBA/1 J小鼠皮内注射II型胶原蛋白和佐剂溶液诱导关节炎，单纯注射佐剂作为对照组。CIA和对照组小鼠在注射后每周处死以评估一系列病理变化。用H&E染色和羟探针1染色观察炎症和缺氧状态。结果在连续的组织病理学观察中，我们可以注意到注射II型胶原后一周滑膜的炎症发展。早在胶原注射后1周，在临床上明显的关节炎之前，也在滑膜中鉴定出通过羟基探针-1染色测量的低血糖变化。此外，我们可以观察到炎症和缺氧共定位于滑膜，并且缺氧的严重程度与滑膜炎的程度呈正相关。结论关节炎前期滑膜组织内存在缺氧，缺氧与滑膜炎的发生有密切的空间关系，且与滑膜炎的严重程度呈正相关。
OBJECTIVE The presence of hypoxia in rheumatoid synovium has been well known, but exact correlation between hypoxia and synovitis is unclear. The aim of our study was to investigate the time and spatial relationship and the correlation of severity between hypoxia and synovitis in pre-arthritic or early stage of inflammatory joint disease. METHODS DBA/1J mice were injected intradermally with type II collagen and adjuvant solution to induce arthritis; mice injected with only adjuvant were used as a control group. CIA and control mice were sacrificed weekly after the injection to evaluate serial pathological changes. H&E stain and hydroxyprobe-1 stain were performed to look at the status of inflammation and hypoxia. RESULTS In serial observations of tissue pathology, we could note the inflammation of synovium developing a week after the injection of type II collagen. Hypoxic change, measured by the hydroxyprobe-1 stain, was also identified in synovium as early as 1 week after the collagen injection, prior to clinically evident arthritis. In addition, we could observe that inflammation and hypoxia co-localize in the synovium and there was a positive correlation between the severity of hypoxia and the degree of synovitis. CONCLUSION Our results demonstrate that hypoxia takes place in synovium at the pre-arthritic stage of disease and have a close spatial relationship and a positive severity correlation with synovitis.