Modulation of potassium channels in human T lymphocytes: effects of temperature.
Modulation of potassium channels in human T lymphocytes: effects of temperature.
复制标题
人 T 淋巴细胞钾通道的调节：温度的影响。
DOI：
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
L. Schlichter
中科院分区：
文献类型：
--
作者：
P. Pahapill;L. Schlichter
1.膜片钳全细胞记录淋巴细胞的主要通道是电压门控性K+通道。有几条证据表明，这些通道与淋巴细胞功能有关。大多数淋巴细胞的功能是温度敏感的，在不同温度下与电生理没有相关性。我们研究了温度对正常人T淋巴细胞电压依赖性K+通道的影响。在5℃到42℃的温度范围内，用全细胞记录研究了宏观电流和单通道事件。2.峰电导、活化率、失活率和失活恢复率都随着温度的升高而递增。在低温条件下，温度对通道开启过程的影响较大。相反，失活过程对室温以上的温度变化最敏感。电导和动力学参数的Arrhenius曲线均为曲线，没有明显的折点。3.37℃时全电池电导的增加既是由于单通道电导的增加，也是由于每个通道在任何时间打开的概率的增加。4.用Hodgkin-Huxley方程和n4j动力学方程对K+电流进行了拟合，得到了所有温度下K+电流的最好描述。5.稳态激活-失活-电压曲线随着升温而向相反方向移动，导致曲线重叠区域更大(窗口电流)。单通道测量证实了更大的窗口电流所预测的静息K+通道活动的增加。6.本研究表明，人T淋巴细胞K+通道的行为具有温度依赖性。
1. The predominant channels found in lymphocytes with patch‐clamp whole‐cell recordings are voltage‐gated K+ channels. Several lines of evidence suggest that these channels are involved in lymphocyte function. Most lymphocyte functions are temperature sensitive and have not been correlated with electrophysiology at different temperatures. We have examined the effect of temperature on the voltage‐dependent K+ channel in normal human T lymphocytes. Both macroscopic current and single‐channel events were studied with whole‐cell recordings at temperatures from 5 to 42 degrees C. 2. Peak conductance, activation rate, inactivation rate and rate of recovery from inactivation all increased progressively as the temperature increased. The effect of temperature on channel opening processes was greater at low temperatures. In contrast, the inactivation process was most sensitive to temperature changes above room temperature. Arrhenius plots of conductance and kinetic parameters were curvilinear with no obvious break‐points. 3. The increase in whole‐cell conductance at 37 degrees C was due to both an increase in the single‐channel conductance and in the probability that each channel is open at any time. 4. K+ currents were fitted by Hodgkin‐Huxley equations with n4j kinetics providing the best description of the currents at all temperatures tested. 5. Steady‐state activation‐ and inactivation‐voltage curves shifted in opposite directions with warming, resulting in a greater area of overlap of the curves ('window' current). The increase in resting K+ channel activity predicted by a greater window current was confirmed with single‐channel measurements. 6. The present study has shown that the behaviour of K+ channels in human T lymphocytes is temperature dependent.
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DOI：
10.1152/ajpcell.1988.254.2.c286
发表时间：
1988
期刊：
The American journal of physiology
影响因子：
--
作者：
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通讯作者：
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DOI：
10.1073/pnas.83.15.5625
发表时间：
1986
影响因子：
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作者：
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DOI：
10.1016/s0006-291x(86)80533-2
发表时间：
1986
影响因子：
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作者：
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DOI：
--
发表时间：
1984
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Trinchieri,G;Perussia,B
通讯作者：
Perussia,B