The nuclear pore complex
The nuclear pore complex
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DOI：
10.4161/nucl.1.1.10571
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发表时间：
2010-01
期刊：
影响因子：
3.7
通讯作者：
C. L. Woodward;S. A. Chow
中科院分区：
文献类型：
--
作者：
C. L. Woodward;S. A. Chow
穿过完整核膜并有效感染非分裂细胞的能力是人类免疫缺陷病毒1型（HIV-1）和其他慢病毒的显著特征，但病毒因子和进入核的机制尚未确定。我们最近报道了核孔蛋白NUP 153在HIV-1前整合复合物（PIC）的核输入中的功能作用。我们的研究结果表明，HIV-1亚病毒颗粒通过PIC相关整合酶（IN）与NUP 153的C-末端结构域的结合直接与核孔复合物（NPC）相互作用而进入核。本文讨论了NPC的构象和构成可能会影响PIC的核输入，以及随后的整合到活跃的转录基因的病毒cDNA。
The ability to traverse an intact nuclear envelope and productively infect non-dividing cells is a salient feature of human immunodeficiency virus type 1 (HIV-1) and other lentiviruses, but the viral factors and mechanism of nuclear entry have not been defined. We have recently reported a functional role for the nucleoporin NUP153 in the nuclear import of the HIV-1 preintegration complex (PIC). Our findings suggest that HIV-1 sub- viral particles gain access to the nucleus by interacting directly with the nuclear pore complex (NPC) via the binding of PIC-associated integrase (IN) to the C-terminal domain of NUP153. This article discusses how NPC conformation and constitution might influence nuclear import of the PIC, and the subsequent integration of the viral cDNA into actively transcribed genes.