Inhibition of metastatic tumor growth in nude mice by portal vein infusions of matrix-targeted retroviral vectors bearing a cytocidal cyclin G1 construct.
Inhibition of metastatic tumor growth in nude mice by portal vein infusions of matrix-targeted retroviral vectors bearing a cytocidal cyclin G1 construct.
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发表时间：
2000-07
期刊：
影响因子：
11.2
通讯作者：
E. Gordon;P. Liu;Z. H. Chen;L. Liu;M. Whitley;C. Gee;S. Groshen;D. Hinton;R. Beart;F. Hall
中科院分区：
文献类型：
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作者：
E. Gordon;P. Liu;Z. H. Chen;L. Liu;M. Whitley;C. Gee;S. Groshen;D. Hinton;R. Beart;F. Hall
肿瘤侵袭和相关的血管生成引起细胞外基质成分的重塑。携带辅助基质靶向基序的逆转录病毒载体（即，胶原蛋白结合多肽）在新暴露的胶原蛋白位点处积累，从而促进肿瘤位点特异性基因递送。在这项研究中，我们评估了一系列的门静脉输注的基质靶向载体携带的突变细胞周期蛋白G1（dnG1）构建在裸鼠模型中的肝转移瘤的抗肿瘤作用。与对照载体或PBS处理的动物相比，dnG 1载体处理的小鼠中肿瘤灶的大小显着减小（P = 0.0002）。这些发现代表了转移性癌症靶向注射载体开发的决定性进展。
Tumor invasion and associated angiogenesis evoke a remodeling of extracellular matrix components. Retroviral vectors bearing auxiliary matrix-targeting motifs (ie., collagen-binding polypeptides) accumulate at sites of newly exposed collagen, thus promoting tumor site-specific gene delivery. In this study, we assessed the antitumor effects of serial portal vein infusions of matrix-targeted vectors bearing a mutant cyclin G1 (dnG1) construct in a nude mouse model of liver metastasis. The size of tumor foci was dramatically reduced in dnG1 vector-treated mice compared with that in control vector- or PBS-treated animals (P = 0.0002). These findings represent a definitive advance in the development of targeted injectable vectors for metastatic cancer.