Effects of vascular endothelial growth factor on osteoclast induction during tooth movement in mice
Effects of vascular endothelial growth factor on osteoclast induction during tooth movement in mice
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DOI：
10.1177/00220345010800100401
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发表时间：
2001-10-01
影响因子：
7.6
通讯作者：
Tanne, K
中科院分区：
文献类型：
--
作者：
Kaku, M;Kohno, S;Tanne, K
对于正畸牙齿移动，牙槽骨的重塑是由骨吸收和新骨形成的重复过程维持的，分别由破骨细胞和成骨细胞控制。最近，我们发现重组人血管内皮生长因子(RhVEGF)作为巨噬细胞集落刺激因子在骨质疏松(OP/OP)小鼠的破骨细胞诱导中发挥作用。本研究的目的是探讨在实验性牙齿移动过程中，重组人血管内皮生长因子是否刺激破骨细胞分化。将纯化的重组人血管内皮生长因子一次性注射到切牙周围的颊牙槽内。将带螺旋环的实验矫治器粘接在上切牙上，施加1.0g的初力，持续3天。牙槽骨受压侧牙周韧带间隙内出现的破骨细胞明显增多。简写：重组人血管内皮细胞生长因子(RhVEGF)、巨噬细胞集落刺激因子(M-CSF)、骨质疏松小鼠(OP/OP小鼠)、鳍状酪氨酸激酶(Flt-1)、牙周膜(PDL)、磷酸盐缓冲液(PBS)、抗酒石酸酸性磷酸酶(TRAP)和方差分析(ANOVA)。
For orthodontic tooth movement, remodeling of the alveolar bone is maintained by a repeated process of bone resorption and new bone formation, controlled, respectively, by osteoclasts and osteoblasts. Recently, we have found that recombinant human vascular endothelial growth factor (rhVEGF) acts as a macrophage colony-stimulating factor in osteoclast induction in osteopetrotic (op/op) mice. The purpose of this study was to investigate whether rhVEGF stimulates osteoclast differentiation during experimental tooth movement. Purified rhVEGF was injected once into the buccal gingival groove around the incisors. An experimental appliance with a helical loop was bonded onto the upper incisors, and an initial force of 1.0 g was applied for three days. The number of osteoclasts appearing in the periodontal ligament space on the pressure side of the alveolar bone was increased markedly. These results suggest that local administration of rhVEGF enhances the number of osteoclasts, and may increase the rate of orthodontic tooth movement.Abbreviations: recombinant human vascular endothelial growth factor (rhVEGF), macrophage colony-stimulating factor (M-CSF), osteopetrotic mice (op/op mice), fins-like tyrosine kinase (Flt-1), periodontal ligament (PDL), phosphate-buffered saline (PBS), tartrate-resistant acid phosphatase (TRAP), and analysis of variance (ANOVA).