Optimization of carbon and energy utilization through differential translational efficiency
Optimization of carbon and energy utilization through differential translational efficiency
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DOI：
10.1038/s41467-018-06993-6
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发表时间：
2018-10-26
影响因子：
16.6
通讯作者：
Zengler, Karsten
中科院分区：
文献类型：
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作者：
Al-Bassam, Mahmoud M.;Kim, Ji-Nu;Zengler, Karsten
控制翻译对所有物种都至关重要。在这里，我们采用多组学的方法来破译条件依赖的翻译调节在模型酵母ijungdahli梭菌。来自自养或异养细胞的综合数据显示，碳和能量代谢的关键途径受到强烈的翻译调节。在不同的生长条件下，参与碳和能量代谢的主要途径不仅转录和翻译存在差异，而且它们的翻译效率也会随着资源的可用性而有所不同。我们发现翻译效率不是静态的，它会随着mRNA表达水平的变化而动态变化。具有优化的5'-非翻译区和编码区特征的mrna具有更高的翻译效率，并且编码碳和能量代谢的基因显著丰富。相比之下，富含管家功能的mrna具有次优特征，翻译效率较低。我们认为，调控转化效率对于有效控制能量匮乏微生物的资源分配至关重要。
Control of translation is vital to all species. Here we employ a multi-omics approach to decipher condition-dependent translational regulation in the model acetogen Clostridium Ijungdahlii. Integration of data from cells grown autotrophically or heterotrophically revealed that pathways critical to carbon and energy metabolism are under strong translational regulation. Major pathways involved in carbon and energy metabolism are not only differentially transcribed and translated, but their translational efficiencies are differentially elevated in response to resource availability under different growth conditions. We show that translational efficiency is not static and that it changes dynamically in response to mRNA expression levels. mRNAs harboring optimized 5'-untranslated region and coding region features, have higher translational efficiencies and are significantly enriched in genes encoding carbon and energy metabolism. In contrast, mRNAs enriched in housekeeping functions harbor suboptimal features and have lower translational efficiencies. We propose that regulation of translational efficiency is crucial for effectively controlling resource allocation in energy-deprived microorganisms.