Clinical Application of a Chromogenic Substrate Method for Determination of Factor VIII Activity
Clinical Application of a Chromogenic Substrate Method for Determination of Factor VIII Activity
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显色底物法测定因子 VIII 活性的临床应用
DOI：
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发表时间：
1985
影响因子：
6.7
通讯作者：
H. Vinazze
中科院分区：
文献类型：
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作者：
S. Rosén;M. Andersson;M. Blombäck;U. Hégglund;M. Larrièu;M. Wolf;C. Boyer;C. Rothschild;I. Nilsson;E. Sjörin;H. Vinazze
摘要用于测定因子VIII活性的显色底物试剂盒(Coatest®因子VIII)已在五个不同的实验室进行了评估，其中一个实验室使用了半自动程序。用发色法和一期凝血法测定健康人、血友病A携带者、重度、轻度和中度血友病A患者以及von Willebrand患者血浆中的凝血因子VIII。在所有这些病例中，这两种方法之间的相关性很高(r=0.96-0.99，n=385)，所有水平的第VIII因子的指定效价都很一致。6例重症血友病患者输注浓缩液或对von Willebrand患者应用DDAVP后，第VIII因子水平也有很好的相关性(r=0.94)。显色法对凝血酶对因子VIII的预激活不敏感，因此在这些情况下也能产生有效的效力分配。发色法的精密度高于一期凝血法(C.V.=2-5%对4-15%)。总之，新的显色底物法已证明适用于血浆和浓缩物中因子VIII的测定。
Summary A chromogenic substrate kit for the determination of factor VIII activity (COATEST® Factor VIII) has been evaluated in five different laboratories, one of them using a semi-automated procedure. This chromogenic method was compared to one-stage clotting assays for factor VIII determination in plasmas from healthy subjects, carriers of hemophilia A, severe, mild and moderate hemophilia A as well as von Willebrand’s patients. In all these cases, a high correlation between these two methods was obtained (r = 0.96-0.99, n = 385) with a good agreement of the assigned potencies at all levels of factor VIII. A good correlation (r = 0.94) was also obtained for the levels of factor VIII after infusion of concentrates in six severe hemophiliacs or after administration of DDAVP to von Willebrand’s patients. The chromogenic method is insensitive to preactivation of factor VIII by thrombin, thus yielding valid potency assignments also in these situations. The precision was higher with the chromogenic method than with the one-stage clotting assays (C.V. = 2-5% vs 4-15%). Altogether, the new chromogenic substrate method has proven itself suitable for determination of factor VIII in plasma and concentrates.