Biological mechanisms of cadmium accumulation in edible Amaranth (Amaranthus mangostanus L.) cultivars promoted by salinity: A transcriptome analysis
Biological mechanisms of cadmium accumulation in edible Amaranth (Amaranthus mangostanus L.) cultivars promoted by salinity: A transcriptome analysis
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DOI：
10.1016/j.envpol.2020.114304
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发表时间：
2020-07-01
影响因子：
8.9
通讯作者：
Zeng, Eddy Y.
中科院分区：
文献类型：
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作者：
Guo, Shi-Hong;Jiang, Ling-Yan;Zeng, Eddy Y.
不同基因型的作物在盐胁迫下对镉（Cd）迁移的调控策略存在差异，但盐胁迫促进不同基因型作物Cd积累的生物学机制尚不清楚。本研究以低镉积累型苋菜品种全红（QH）和高镉积累型苋菜品种柳叶（LY）为材料，研究了盐胁迫下苋菜镉积累的生物学机制。以LY和QH为材料，分别在交换性Cd 0.27mg kg（-1）和盐3.0g kg（-1）处理和对照条件下，对叶和根组织进行转录组分析。共鉴定出3224个LY（根1119个，叶2105个）和848个QH（根207个，叶641个）差异表达基因。几乎在2-2（5）、25-2（10）、>2（10）的倍数变化范围内，LY处理诱导的DEG数量均显著高于QH处理，表明LY对盐敏感性更强。基因本体（GO）分析表明，LY胁迫通过提高根中腺嘌呤代谢相关基因（84倍富集）和质子泵ATP酶相关基因（50倍富集）以及叶片中碳水化合物水解相关基因（2.5倍富集）的表达，促进了土壤酸化和Cd的活化。有机酸转运蛋白（ALMT）基因在根中的表达提高了2.71 ~ 3.94倍，促进了有机酸的分泌。盐胁迫还抑制了根细胞壁生物合成相关关键酶的表达，降低了镉吸收的物理障碍。所有这些过程中改变LY更显着相比，QH，表明盐敏感品种可能积累更多的镉，并构成更高的健康风险。(C)2020爱思唯尔有限公司保留所有权利。
Strategies to prevent cadmium (Cd) mobilization by crops under salinity conditions differs among distinct genotypes, but the biological mechanisms of Cd accumulation in different genotype crops promoted by salinity have remained scarce. In this study, we investigated the biological mechanisms of Cd accumulation in two quite different amaranth cultivars of low-Cd accumulator Quanhong (QH) and highCd accumulator Liuye (LY) in response to salt stress. Transcriptomes analysis was carried out on leaves and roots tissues of LY and QH grown with exchangeable Cd 0.27 mg kg(-1) and salinity 3.0 g kg(-1) treatment or control conditions, respectively. A total of 3224 differentially expressed genes (DEGs) in LY (1119 in roots, 2105 in leaves) and 848 in QH (207 in roots, 641 in leaves) were identified. Almost in each fold change category (2-2(5), 25-2(10), >2(10)), the numbers of DEGs induced by salinity in LY treatments were much more than those in QH treatments, indicating that LY is more salt sensitive. Gene ontology (GO) analysis revealed that salinity stress promoted soil acidification and Cd mobilization in LY treatments through the enhancive expression of genes related to adenine metabolism (84-fold enrichment) and proton pumping ATPase (50-fold enrichment) in roots, and carbohydrate hydrolysis (2.5-fold enrichment) in leaves compared with that of whole genome, respectively. The genes expression of organic acid transporter (ALMT) was promoted by 2.71- to 3.94-fold in roots, facilitating the secretion of organic acids. Salt stress also inhibited the expression of key enzymes related to cell wall biosynthesis in roots, reducing the physical barriers for Cd uptake. All these processes altered in LY were more substantially compared with that of QH, suggesting that salt sensitive cultivars might accumulate more Cd and pose a higher health risk. (C) 2020 Elsevier Ltd. All rights reserved.