Preparation and in vitro analysis of microencapsulated live Lactobacillus fermentum 11976 for augmentation of feruloyl esterase in the gastrointestinal tract
Preparation and in vitro analysis of microencapsulated live Lactobacillus fermentum 11976 for augmentation of feruloyl esterase in the gastrointestinal tract
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DOI：
10.1042/ba20070007
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发表时间：
2008-05-01
影响因子：
2.8
通讯作者：
Prakash, Satya
中科院分区：
文献类型：
--
作者：
Bhathena, Jasmine;Kulamarva, Arun;Prakash, Satya
FA（阿魏酸）是存在于植物细胞壁中的众所周知的酚类植物化学物质。各种研究表明，FA在预防慢性疾病方面具有许多生理功能。它已被证明在退行性疾病中发挥重要的化学保护作用。FA还显示出强大的抗氧化和亚硝酸盐清除潜力，抗癌和抗炎特性。FA的体内生理重要性取决于其吸收的可用性。膳食纤维结合的FA部分由肠道微器官释放;然而，释放的FA的浓度太低而不能作为化学预防剂。因此，增加FA的生物利用度以更充分地了解其真实的生理效应是重要的。本文评价了海藻酸盐-聚（L-赖氨酸）-海藻酸盐微胶囊的适用性，口服活阿魏酸酯酶生产发酵乳杆菌11976细胞，在体外，通过使用动态模拟人GI（胃肠道）模型。本研究表明，微胶囊L。Fermentum 11976细胞可以有效地分解含FA的底物，并建立了它们用于补充肠道中膳食FA的生物利用度的生物技术基础。
FA (ferulic acid) is a well-known phenolic phytochemical present in plant cell walls. Various studies have indicated that FA has many physiological functions in the prevention of chronic disease. It has been shown to play an important chemoprotective role in degenerative diseases. FA also shows strong antioxidant and nitrite-scavenging potential and anticarcinogenic and anti-inflammatory properties. The in vivo physiological importance of FA depends on its availability for absorption. Dietary fibre-bound FA is partially released by gut micro-organ isms; however, the concentration of the released FA is too low to act as a chemopreventive agent. Therefore it is important to augment the bioavailability of FA to appreciate more fully its real physiological effect. This paper evaluates the suitability of the alginate-poly(L-lysine)-alginate microcapsules for oral delivery of live feruloyl esterase-producing Lactobacillus fermentum 11976 cells, in vitro, by using a dynamic simulated human GI (gastrointestinal) model. The present study shows that microencapsulated L. fermentum 11976 cells can efficiently break down a FA-containing substrate, and establishes the biotechnological basis for their use in supplementing the bioavailability of dietary FA in the intestine.