Mycobacterium vicinigordonae sp. nov., a slow-growing scotochromogenic species isolated from sputum
Mycobacterium vicinigordonae sp. nov., a slow-growing scotochromogenic species isolated from sputum
复制标题
分枝杆菌 vicinigordonae sp.
DOI：
10.1099/ijsem.0.004796
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Hairong Huang
中科院分区：
文献类型：
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作者：
Guan Liu;Xia Yu;Jingjign Luo;Yanjie Hu;Lingling Dong;Guanglu Jiang;Fengmin Huo;Fen Wang;Qian Liang;Hairong Huang
从一名中国男性的痰液中分离出一种生长缓慢、暗色的分枝杆菌菌株 (24T)。使用16S rRNA基因进行系统发育分析，将菌株24T指定为戈登分枝杆菌复合体，其中包括戈登分枝杆菌和副戈登分枝杆菌。表型特征、独特的分枝菌酸谱和基于hsp65和rpoB序列的系统发育分析结果有力地支持了菌株24Ta的分类地位，它是与M其他成员不同的物种的代表。戈登复合体。菌株24T基因组G+C含量为65.40mol%。基因组比较表明菌株 24TandM。 gordonaeATCC 14470T的平均核苷酸同一性（ANI）值为81.00 ％，DNA-DNA杂交（DDH）值为22.80 ％，而菌株24T和M之间的ANI和DDH值。 paragordonae49 061T 分别为 80.98 和 22.80 %。在系统发育、表型和化学分类学特征方面，菌株24Ti与其最接近的系统发育亲属有区别，代表分枝杆菌属的一个新种，因此被命名为Mycobacter vicinigordonaesp。十一月被提议。模式菌株为24T(=CMCC 93559T=DSM 105979T)。
A slow-growing, scotochromogenic mycobacterial strain (24T) was isolated from the sputum of a Chinese male human. Phylogenetic analysis using the 16S rRNA gene assigned strain 24Tto theMycobacterium gordonaecomplex, which includesMycobacterium gordonaeandMycobacterium paragordonae. The phenotypic characteristics, unique mycolic acid profile and the results of phylogenetic analysis based onhsp65andrpoBsequences strongly supported the taxonomic status of strain 24Tas a representative of a species distinct from the other members of theM. gordonaecomplex. The genomic G+C content of strain 24Twas 65.40mol%. Genomic comparisons showed that strain 24TandM. gordonaeATCC 14470Thad an average nucleotide identity (ANI) value of 81.00 % and a DNA–DNA hybridization (DDH) value of 22.80 %, while the ANI and DDH values between strain 24TandM. paragordonae49 061Twere 80.98 and 22.80 %, respectively. In terms of phylogenetic, phenotypic and chemotaxonomic features, strain 24Tis distinguishable from its closest phylogenetic relatives and represents a novel species of the genusMycobacterium, therefore the nameMycobacterium vicinigordonaesp. nov. is proposed. The type strain is 24T(=CMCC 93559T=DSM 105979T).