Cortical sinus probing, S1P1-dependent entry and flow-based capture of egressing T cells.
Cortical sinus probing, S1P1-dependent entry and flow-based capture of egressing T cells.
复制标题
作者：
淋巴细胞从淋巴结流出的细胞动力学尚未明确界定。在此，我们观察到淋巴结皮质窦的分支结构，并且发现一些T细胞在进入后被滞留，而其他T细胞则返回实质。1 - 磷酸鞘氨醇受体1（S1P1）缺陷型T细胞探测窦表面但无法进入。在一些窦中，T细胞变圆并单向移动。T细胞从皮质窦进入富含巨噬细胞的窦区域。许多T细胞从髓窦流入被膜下间隙。我们提出了一个淋巴结流出的多步骤模型，即首先是皮质窦探测，接着是依赖S1P1的进入，细胞在有血流的窦区域被捕获，然后运输到髓窦和输出淋巴管。
The cellular dynamics of lymphocyte egress from lymph nodes are poorly defined. Here, we visualized the branched organization of lymph node cortical sinuses and found that after entry some T cells were retained while others returned to the parenchyma. Sphingosine-1-phosphate receptor 1 (S1P1)-deficient T cells probed the sinus surface but failed to enter. In some sinuses T cells became rounded and moved in a unidirectional fashion. T cells traveled from cortical sinuses into macrophage-rich sinus areas. Many T cells flowed from medullary sinuses into the subcapsular space. We propose a multistep model of lymph node egress where cortical sinus probing is followed by S1P1-dependent entry, capture of cells in a sinus region with flow and transport to medullary sinuses and the efferent lymph.