Whole-cell currents in olfactory receptor cells of Xenopus laevis
Whole-cell currents in olfactory receptor cells of Xenopus laevis
复制标题
非洲爪蟾嗅觉受体细胞的全细胞电流
DOI：
10.1007/bf00228894
复制
发表时间：
2004
影响因子：
2
通讯作者：
Detlev Schild
中科院分区：
文献类型：
--
作者：
Detlev Schild
本研究采用非酶消化法分离非洲爪蟾（Xenopus laevis）的嗅粘膜，并对分离的嗅神经元进行膜片钳全细胞记录。在电压钳中，可以区分五种电流成分：快速的TTX敏感的Na+电流，由Ca 2+离子携带的小而慢的内向电流，Ca 2+依赖的K+电流，在大于-20 mV的正电压下快速激活并快速失活的K+电流，以及缓慢激活和非常缓慢失活的K+电流。本文报道的全细胞电流的一些特征与以前的研究结果相矛盾，而另一些则验证了它们，从而允许对它们在转导过程中的生理作用进行初步解释。
SummaryOlfactory mucosae of Xenopus laevis were dissociated without enzymatic treatment and the isolated olfactory neurones were studied with the tight-seal whole-cell recording configuration of the patch clamp technique. In the voltage clamp, five current components could be distinguished: a fast, TTX-sensitive Na+-current, a small and slow inward current carried by Ca2+ ions, a Ca2+ dependent K+-current, a K+-current which activates rapidly at voltages more positive than-20 mV and quickly inactivates, and a slowly activating and very slowly inactivating K+-current. Some of the characteristics of the whole-cell currents herein reported contradict previous findings while others verify them, thereby allowing a tentative interpretation of their physiological role in the transduction process.